For immunochemical detection of Doppel
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Doppel Polyclonal Antibody

Item No. 10005517

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • DPL
  • Prion-like Protein Doppel
  • Prion Protein 2
  • PRND
  • PrPLP
Immunogen
Synthetic peptide from an internal region of human Doppel
Formulation (Request formulation change)
500 µl of peptide affinity-purified polyclonal antibody
Storage Buffer
TBS, pH 7.4, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide
Host
Rabbit
Applications
IHC and WB
Species Reactivity
(+) Human(+) Mouse(+) Rat
UniProt Accession №
Q9UKY0
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea (500 µl)

    $347

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    Product Description

    Doppel is a homolog of the cellular prion protein. Like prion protein, doppel has two N-linked oligosaccharides, and is presented on the cell surface via a glycosylphosphatidylinositol anchor.1 Unlike prion protein, it lacks the conformationally plastic and octapeptide repeat domains.2 The primary physiological role of doppel protein remains to be determined, but there is some evidence suggesting that cell surface prion protein can antagonize the toxic effect of doppel expressed in the central nervous system.1 In addition, the protein may play a major role in human male fertility, given its expression on both sertoli cells and spermatozoa.3 Doppel is differentially glycosylated, causing it to migrate at multiple sizes on SDS-PAGE.1 Cayman’s Doppel Polyclonal Antibody can be used for immunohistochemistry and Western blot applications. The antibody recognizes Doppel at 19 kDa (nonglycosylated) and 25 kDa (monoglycosylated) from human, mouse, and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Silverman, G.L., Qin, K., Moore, R.C., et al. Doppel is an N-glycosylated, glycosylphosphatidylinositol-anchored protein. Expression in testis and ectopic production in the brains of Prnp0/0 mice predisposed to purkinje cell loss. The Journal of Biological Chemisty 275(35), 26834-26841 (2000).

    2. Mastrangelo, P., and Westaway, D. The prion gene complex encoding PrPc and Doppel: Insights from mutational analysis. Gene 275, 1-18 (2001).

    3. Peoc'h, K., Serres, C., Frobert, Y., et al. The human 'prion-like' protein doppel is expressed in both sertoli cells and spermatozoa. The Journal of Biological Chemisty 277(45), 43071-43078 (2002).