A selective, fluorescent probe for H2O2 detection
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Pentafluorobenzenesulfonyl fluorescein

Item No. 10005983

Technical Information
Formal Name
pentafluorobenzenesulfonic acid, 6’-hydroxy-3-oxospiro[isobenzofuran-1(3H),9’-[9H]xanthen]-3’-yl ester
CAS Number
728912-45-6
Molecular Formula
C26H11F5O7S
Formula Weight
Purity
≥98%
Emission
530 ±25 nm
Excitation
485 ±20 nm
A crystalline solid
DMF: 30 mg/mlDMSO: 30 mg/mlDMSO:PBS (pH 7.2)(1:1): .25 mg/mlEthanol: 20 mg/ml
SMILES
FC1=C(F)C(F)=C(F)C(F)=C1S(OC2=CC=C(C3(OC(C4=CC=CC=C43)=O)C(C=CC(O)=C5)=C5O6)C6=C2)(=O)=O
InChi Code
InChI=1S/C26H11F5O7S/c27-19-20(28)22(30)24(23(31)21(19)29)39(34,35)38-12-6-8-16-18(10-12)36-17-9-11(32)5-7-15(17)26(16)14-4-2-1-3-13(14)25(33)37-26/h1-10,32H
InChi Key
UIBLLPHZQUTIBL-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    Pentafluorobenzenesulfonyl fluorescein is a fluorescent probe that functions by a nonoxidative mechanism. It fluoresces upon perhydrolysis of the sulfonyl linkage, and is selective for hydrogen peroxide over hydroxyl radical, tert-butyl hydroperoxide, superoxide anion, singlet oxygen, and nitrates. Pentafluorobenzenesulfonyl fluorescein can be used as a probe for hydrogen peroxide in whole cell systems.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Maeda, H., Fukuyasu, Y., Yoshida, S., et alFluorescent probes for hydrogen peroxide based on a non-oxidative mechanism. Angew. Chem. Int. Ed. Engl. 43(18), 2389-2391 (2004).