DMPO Nitrone Adduct Polyclonal Antiserum

DMPO is a spin trap that reacts with oxygen-, nitrogen-, sulfur-, and carbon-centered radicals.^1,2,3 It also reacts with DNA base- and amino acid-derived radicals to form DNA- and protein-DMPO nitroxone adducts, respectively, which rapidly decay to form DMPO-nitrone adducts.⁴ DMPO-nitrone adducts were previously detected using electron paramagnetic resonance (EPR), however, immunochemical detection allows a higher level of sensitivity and throughput with greatly reduced sample consumption.^4,5,6 Cayman’s DMPO Nitrone Adduct Polyclonal Antiserum can be used for Western blot, immunocytochemistry, and ELISA applications. The antiserum recognizes various DMPO-nitrone adducts, including DNA and protein species. This material was produced using unpurified rabbit serum directed against an ovalbumin-DMPO conjugate and its use may result in off-target reactivity to similar protein epitopes as those found within the antigen.

WARNING This product is not for human or veterinary use.

1. Chamulitrat, W., Jordan, S.J., and Mason, R.P. Fatty acid radical formation in rats administered oxidized fatty acids: In vivo spin trapping investigation. Arch. Biochem. Biophys. 299(2), 361-367 (1992).

2. Dikalov, S., Kirilyuk, I., and Grigor'ev, I. Spin trapping of O-,C-, and S-centered radicals and peroxynitrite by 2H-imidazole-1-oxides. Biochem. Biophys. Res. Commun. 218(2), 616-622 (1996).

3. Kalyanaraman, B., Karoui, H., Singh, R.J., et al. Detection of thiyl radical adducts formed during hydroxyl radical- and peroxynitrite-mediated oxidation of thiols - a high resolution ESR spin-trapping study at Q-band (35GHz). Anal. Biochem. 241(1), 75-81 (1996).

4. Mason, R.P. Imaging free radicals in organelles, cells, tissue, and in vivo with immuno-spin trapping. Redox Biol. 8, 422-429 (2016).

5. Ramirez, D.C., Chen, Y.R., and Mason, R.P. Immunochemical detection of hemoglobin-derived radicals formed by reaction with hydrogen peroxide: Involvement of a protein-tyrosyl radical. Free Radic. Biol. Med. 34(7), 830-839 (2003).

6. Towner, R.A., and Smith, N. In vivo and in situ detection of macromolecular free radicals using immuno-spin trapping and molecular magnetic resonance imaging. Antioxid. Redox Signal. 28(15), 1404-1415 (2018).