For immunochemical detection of S1P3
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S1P3 Polyclonal Antibody

Item No. 10006373

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • EDG-3
  • S1PR3
  • Sphingosine-1-phosphate Receptor 3
Immunogen
Synthetic peptide from the N-terminal region of human S1P3
Amino Acids
12-25
Formulation (Request formulation change)
Peptide affinity-purified antibody lyophilized from 500 µl
Storage Buffer
TBS, pH 7.4 with 5 mg/ml BSA when reconstituted with 500 µl double distilled water
Host
Rabbit
Applications
ICC and WB
Species Reactivity
(+) Human(+) Mouse(+) Rat
UniProt Accession №
Q99500
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea

    $347

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    Product Description

    Sphingosine-1-phosphate (S1P) exerts its activity by binding to five distinct G-protein-coupled receptors, S1P1/EDG-1, S1P2/EDG-5, S1P3/EDG-3, S1P4/EDG-6, and S1P5/EDG-8.1,2 S1P3 couples to Gi/o-ERK, Gq-PLC, and G12/13-Rho axes to mediate S1P-induced cell proliferation, survival, migration, and related signaling events.1,2,3 S1P3 is widely expressed in various tissues, suggesting diverse physiological functions of this receptor.4 The human and mouse S1P3 have 378 amino acids with an estimated molecular weight of 42 kDa. Glycosylation at the N-terminal extracellular domain may cause the protein to migrate at different positions in SDS-PAGE. Cayman’s S1P3 Polyclonal Antibody can be used for immunocytochemistry and Western blot applications. The antibody recognizes S1P3 at 47 kDa from human, mouse, and rat samples. Liver and heart tissues display 2-3 bands between 40-50 kDa, possibly due to different degree of glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Takuwa, Y., Takuwa, N., and Sugimoto, N. The Edg family G protein-coupled receptors for lysophospholipids: Their signaling properties and biological activities. J. Biochem. 131(6), 767-771 (2002).

    2. Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu. Rev. Biochem. 73, 321-354 (2004).

    3. Kluk, M.J., and Hla, T. Signaling of sphingosine-1-phosphate via the S1P/EDG-family of G-protein-coupled receptors. Biochim. Biophys. Acta 1582(1-3), 72-80 (2002).

    4. Takuwa, Y. Subtype-specific differential regulation of Rho family G proteins and cell migration by the Edg family sphingosine-1-phosphate receptors. Biochim. Biophys. Acta 1582, 112-120 (2002).

    Product Citations

    Fischer, I., Alliod, C., Martinier, N., et al. Sphingosine kinase 1 and sphingosine 1-phosphate receptor 3 are functionally upregulated on astrocytes under pro-inflammatory conditions. PLoS One 6(8), e23905 (2011).