Commonly-used spin trap
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DMPO

Item No. 10006436

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3,4-dihydro-2,3-dimethyl-2H-pyrrole 1-oxide
CAS Number
3317-61-1
Synonyms
  • 5,5-Dimethyl-1-Pyrroline-N-Oxide
Molecular Formula
C6H11NO
Formula Weight
113.2
Purity
≥98%
Formulation (Request formulation change)
A liquid
Solubility(Learn about Variance in Solubility)
DMF: 42 mg/mlDMSO: 50 mg/mlEthanol: 50 mg/mlPBS (pH 7.2): 9.2 mg/ml
λmax
235 nm
SMILES
O[N]1=CCCC1(C)C
InChi Code
InChI=1S/C6H11NO/c1-6(2)4-3-5-7(6)8/h5H,3-4H2,1-2H3
InChi Key
VCUVETGKTILCLC-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    500 mg

    $72

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    1 g

    $137

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    5 g

    $501

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    Product Description

    DMPO is a commonly-used spin trap that reacts with O-, N-, S-, and C-centered radicals.1,2,3 This allows their characterization when used in association with electron spin resonance and immuno-spin trapping.4,5,6 DMPO is water-soluble, rapidly penetrates lipid bilayers, has low toxicity, and can be used in vitro and in vivo.7,8,9

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Chamulitrat, W., Jordan, S.J., and Mason, R.P. Fatty acid radical formation in rats administered oxidized fatty acids: In vivo spin trapping investigation. Arch. Biochem. Biophys. 299(2), 361-367 (1992).

    2. Dikalov, S., Kirilyuk, I., and Grigor'ev, I. Spin trapping of O-,C-, and S-centered radicals and peroxynitrite by 2H-imidazole-1-oxides. Biochem. Biophys. Res. Commun. 218(2), 616-622 (1996).

    3. Kalyanaraman, B., Karoui, H., Singh, R.J., et al. Detection of thiyl radical adducts formed during hydroxyl radical- and peroxynitrite-mediated oxidation of thiols - a high resolution ESR spin-trapping study at Q-band (35GHz). Anal. Biochem. 241(1), 75-81 (1996).

    4. Mason, R.P. Using anti-5,5-dimethyl-1-pyrroline N-oxide (anti-DMPO) to detect protein radicals in time and space with immuno-spin trapping. Free Radic. Biol. Med. 36(10), 1214-1223 (2004).

    5. Ramirez, D.C., Mejiba, S.E.G., and Mason, R.P. Mechanism of hydrogen peroxide-induced Cu,Zn-superoxide dismutase-centered radical formation as explored by immuno-spin trapping: The role of copper- and carbonate radical anion-mediated oxidations. Free Radic. Biol. Med. 38(2), 201-214 (2004).

    6. Detweiler, C.D., Deterding, L.J., Tomer, K.B., et al. Immunological identification of the heart myoglobin radical formed by hydrogen peroxide. Free Radic. Biol. Med. 33(3), 364-369 (2002).

    7. Anzai, K., Aikawa, T., Furukawa, Y., et al. ESR measurement of rapid penetration of DMPO and DEPMPO spin traps through lipid bilayer membranes. Arch. Biochem. Biophys. 415(2), 251-256 (2003).

    8. Haseloff, R.F., Mertsch, K., Rohde, E., et al. Cytotoxicity of spin trapping compounds. FEBS Lett. 418(1-2), 73-75 (1997).

    9. Jiang, J., Liu, K.J., Jordan, S.J., et al. Detection of free radical metabolite formation using in vivo EPR spectroscopy: Evidence of rat hemoglobin thiyl radical formation following administration of phenylhydrazine. Arch. Biochem. Biophys. 330(2), 266-270 (1996).