For immunochemical detection of SPHK 1
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Applicable for Use with
10006823Sphingosine Kinase 1 Blocking Peptide
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Sphingosine Kinase 1 Polyclonal Antibody

Item No. 10006822

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • SK 1
  • SPHK 1
  • SPK 1
Immunogen
Synthetic peptide from an internal region of human SPHK 1
Formulation (Request formulation change)
Lyophilized peptide affinity-purified antibody
Storage Buffer
TBS, pH 7.4, with 5 mg/ml BSA when reconstituted in 500 μl double distilled water
Host
Rabbit
Applications
ICC and WB; other applications not tested
Species Reactivity
(+) Human(+) Mouse(+) Pig; other species not tested
UniProt Accession №
Q9NYA1 (human)
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    SPHK 1 catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate. This reaction plays an important role in determining cell proliferation versus cell death.1,2 SPHK 1 is found in a wide variety of tissues and cell types including kidney, liver, spleen, heart, platelets, and human tumors. On a cellular level, it is found in the cytosolic and membrane fractions.3 Based on the amino acid sequence, this protein has a molecular weight of approximately 43 kDa. The observed band at 50 kDa may be explained by some reported post translational modifications.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Delon, C., Manifava, M., Wood, E., et al. Sphingosine kinase 1 is an intracellular effector of phosphatidic acid. The Journal of Biological Chemisty 279(43), 44763-44774 (2004).

    2. Wang, E., Norred, W.P., Bacon, C.W., et al. Inhibition of sphingolipid biosynthesis by fumonisins. Implications for diseases associated with Fusarium moniliforme. The Journal of Biological Chemisty 266(22), 14486-14490 (1991).

    3. Maceyka, M., Sankala, H., Hait, N.C., et al. SphK1 and SphK2, sphingosine kinase isoenzymes with opposing functions in sphingolipid metabolism. The Journal of Biological Chemisty 280(44), 37118-37129 (2005).

    4. Pitson, S.M., D'Andrea, R.J., Vandeleur, L., et al. Human sphingosine kinase: Purification, molecular cloning and characterization of the native and recombinant enzymes. Biochem. J. 350(Pt 2), 429-441 (2000).