CRTH2/DP₂ Receptor (C-Term) Polyclonal Antibody

Prostaglandin D₂ (PGD₂) elicits its biological function through interaction with two distinct G protein-coupled receptors, DP₁ and CRTH2/DP₂. CRTH2 primarily couples to G_i or G_q subunits to mobilize Ca²⁺, induce cell migration, and up-regulate adhesion molecules.¹ CRTH2 mRNA has been detected in various tissues including liver, lung, kidney, brain, heart, thymus, and spleen and in various cell lineages including both hematopoietic and non-hematopoietic cell lines.² Human CRTH2 is 395 amino acids in length with an estimated molecular weight of 43 kDa. Cayman’s CRTH2/DP₂ receptor (C-Term) polyclonal antibody can be used for western blot of CRTH2 on samples of human, mouse, and rat origin. It detects both unglycosylated and glycosylated protein at sizes ranging from 35-40 to 50-70 kDa, as reported by Nagata et al., in 1999.³

WARNING This product is not for human or veterinary use.

1. Nagata, K., and Hirai, H. The second PGD₂ receptor CRTH2: Structure, properties, and functions in leukocytes. Prostaglandins Leukot. Essent. Fatty Acids 69, 169-177 (2003).

2. Abe, H., Takeshita, T., Nagata, K., et al. Molecular cloning, chromosome mapping and characterization of the mouse CRTH2 gene, a putative member of the leukocyte chemoattractant receptor family. Gene 227(1), 71-77 (1999).

3. Nagata, K., Tanaka, K., Ogawa, K., et al. Selective expression of a novel surface molecule by human Th2 cells in vivo. J. Immunol. 162, 1278-1286 (1999).