Prostaglandin D Synthase (lipocalin-type; human) ELISA Kit

Prostaglandin D synthase (PGDS) catalyzes the isomerization of PGH₂ to produce PGD₂, a potent lipid mediator involved in sleep regulation, nociception, platelet aggregation, and allergic and inflammatory responses. Lipocalin-type PGDS (a.k.a. β-trace) is a homodimer with each subunit ranging in size from 20-31 kDa, depending on the extent of glycosylation. L-PGDS has two functions: it catalyzes the conversion of PGH₂ to PGD₂ and acts as a carrier protein for lipid-like molecules (i.e., retinoids and thyroid hormones).¹ L-PGDS is present in a variety of body fluids including cerebrospinal fluid, seminal fluid, and plasma. Cayman’s L-PGDS (human) ELISA is an immunometric (i.e., sandwich) ELISA with a standard curve ranging from 1.56-100 ng/ml and a limit of detection of 1.56 ng/ml. Inter- and intra-assay CV’s of less than 15% may be achieved at most concentrations. This assay has been validated using cerebrospinal fluid which contains approximately 12-30 µg/ml of L-PGDS.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

1. Urade, Y., and Hayaishi, O. Biochemical, structural, genetic, physiological, and pathophysiological features of lipocalin-type prostaglandin D synthase. Biochim. Biophys. Acta 1482, 259-271 (2000).