A novel, chromogenic substrate for PLC
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PLC thio-PIP2 (sodium salt)

Item No. 10007839

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3-(3-(1,2-dihydroxy-1,2-dihexanoyl)thiopropane)inositol-4,5-diphosphate, trisodium salt
Molecular Formula
C21H38O18P3S • 3Na
Formula Weight
772.5
Purity
≥98%
Formulation (Request formulation change)
A lyophilized powder
SMILES
CCCCCC(OC[C@@H](OC(CCCCC)=O)CSP([O-])(O[C@@H]1[C@H](O)[C@H](O)[C@@H](OP(O)([O-])=O)[C@H](OP(O)([O])=O)[C@H]1O)=O)=O.[Na+].[Na].[Na+]
InChi Code
InChI=1S/C21H41O18P3S.3Na/c1-3-5-7-9-14(22)35-11-13(36-15(23)10-8-6-4-2)12-43-42(33,34)39-19-16(24)17(25)20(37-40(27,28)29)21(18(19)26)38-41(30,31)32;;;/h13,16-21,24-26H,3-12H2,1-2H3,(H,33,34)(H2,27,28,29)(H2,30,31,32);;;/q;3*+1/p-3/t13-,16-,17+,18+,19-,20-,21-;;;/m1.../s1
InChi Key
JEAWYKPPKVXGTD-GQUCJIMCSA-K
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    100 µg

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    Product Description

    Phospholipase C (PLC) catalyzes the hydrolysis of phosphatidylinositol (4,5) bisphosphate (PIP2) on the cytoplasmic side of the cell membrane to yield diacylglycerol and inositol-1,4,5-triphosphate (IP3).1,2,3 This reaction initiates a well-known signal transduction pathway common to most cells. PLC thio-PIP2 is an analog of naturally occurring PIP2 that contains sulfur rather than oxygen at the sn-3 position of the glycerol backbone. Hydrolysis by PLC yields a free thiol which reacts with chromogenic reagents such as DTNB (Ellman’s reagent) to allow quantitation of PLC activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Exton, J.H. Regulation of phosphoinositide phospholipases by hormones, neurotransmitters, and other agonists linked to G proteins. Annu. Rev. Pharmacol. Toxicol. 36, 481-509 (1996).

    2. Exton, J.H. Cell signalling through guanine-nucleotide-binding regulatory proteins (G proteins) and phospholipases. Eur. J. Biochem. 243, 10-20 (1997).

    3. Majerus, P.W. Inositol phosphate biochemistry. Annu. Rev. Biochem. 61, 225-250 (1992).