Convenient 96 well method for detection of TrxR
Features
  • Measure TrxR activity in tissue homogenates and cell lysates
  • Assay 22 samples in duplicate
  • Measure TrxR down to 0.08 U/ml
  • Plate-based colorimetric measurement (405-414 nm)
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Thioredoxin Reductase Colorimetric Assay Kit

Item No. 10007892

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • TrxR
  • TxnR
Assay Range
0.008-0.09 µmol/min/ml
Origin
Animal/Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $329

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    Cayman’s Thioredoxin Reductase Colorimetric Assay Kit provides a convenient method for detecting TrxR activity in tissue homogenates and cell lysates. It is based on the reduction of DTNB (5,5’-dithio-bis(2-dinitrobenzoic acid); Ellman’s reagent) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) which produces a yellow product that is measured at 405 nm.1,2 The kit includes all reagents needed to assay mammalian TrxR activity. Measurement of TrxR activity by DTNB reduction in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction (i.e., presence of glutathione).3 The difference between the two results is the DTNB reduction due to TrxR activity.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Smith, A.D., Morris, V.C., and Levander, O.A. Rapid determination of glutathione peroxidase and thioredoxin reductase activities using a 96-well microplate format: Comparison to standard cuvette-based assays. Int. J. Vitam. Nutr. Res. 71(1), 87-92 (2001).

    2. Hill, K.E., McCollum, G.W., and Burk, R.F. Determination of thioredoxin reductase activity in rat liver supernatant. Anal. Biochem. 253, 123-125 (1997).

    3. Smith, A.D., Guidry, C.A., Morris, V.C., et al. Aurothioglucose inhibits murine thioredoxin reductase activity in vivo. J. Nutr. 129, 194-198 (1999).

    Product Citations

    Alshabibi, M.A., Khatlani, T., Abomaray, F.M., et al. Human decidua basalis mesenchymal stem/stromal cells protect endothelial cell functions from oxidative stress induced by hydrogen peroxide and monocytes. Stem Cell Res. Ther. 9:275, (2018).

    Pan, H., Jansson, K.H., Beshiri, M.L., et al. Gambogic acid inhibits thioredoxin activity and induces ROS-mediated cell death in castration-resistant prostate cancer. Oncotarget 8(44), 77181-77194 (2017).

    Liu, C., Liu, Z., Li, M., et al. Enhancement of auranofin-induced apoptosis in MCF-7 human breast cells by selenocystine, a synergistic inhibitor of thioredoxin reductase. PLoS One 8(1), e53945 (2013).