Prostaglandin F Synthase (human, recombinant)

Human prostaglandin F synthase (PGFS) is a ~37 kDa monomeric protein with high sequence similarity to aldo-keto reductase family members.¹ It exhibits two related primary activities, catalyzing the isomerization of PGH₂ to PGF_2α (PGH₂ 9,11-endoperoxide reductase activity), as well as the formation of 11β-PGF_2α from PGD₂ (PGD₂ 11-ketoreductase activity) in the presence of NADPH. The enzyme also exhibits reductase activities toward carbonyl compounds such at nitroacetophenone, nitrobenzaldehyde, and 9,10-phenanthrene quinine (PQ).² Cayman’s human recombinant PGFS has been expressed and purified from an E.coli overexpression system. Enzyme activity was determined by monitoring the change in absorbance at 340 nm using PGD₂ as the substrate and NADPH as the cofactor (PGD₂ 11-ketoreductase activity).

WARNING This product is not for human or veterinary use.

1. Urade, Y., Watanabe, K., and Hayaishi, O. Prostaglandin D, E, and F synthases. J. Lipid Mediat. Cell Signal. 12(2-3), 257-273 (1995).

2. Suzuki, T., Fuji, Y., Miyano, M., et al. cDNA cloning, expression, and mutagenesis study of liver-type prostaglandin F synthase. The Journal of Biological Chemisty 274, 241-248 (1999).