A fluorescent long-chain ceramidase substrate
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C12 NBD Ceramide (d18:1/12:0)

Item No. 10007958

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[(1S,2R,3E)-2-hydroxy-1-(hydroxymethyl)-3-heptadecen-1-yl]-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-dodecanamide
CAS Number
202850-01-9
Synonyms
  • NBD Cer(d18:1/12:0)
  • NBD Ceramide (d18:1/12:0)
  • N-C12-NBD-D-erythro-Sphingosine
Molecular Formula
C36H61N5O6
Formula Weight
659.9
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 20 mg/mlEthanol: 20 mg/ml
λmax
333, 465 nm
SMILES
OC[C@H](NC(CCCCCCCCCCCNC1=CC=C([N+]([O-])=O)C2=NON=C12)=O)[C@H](O)/C=C/CCCCCCCCCCCCC
InChi Code
InChI=1S/C36H61N5O6/c1-2-3-4-5-6-7-8-9-10-12-15-18-21-24-33(43)31(29-42)38-34(44)25-22-19-16-13-11-14-17-20-23-28-37-30-26-27-32(41(45)46)36-35(30)39-47-40-36/h21,24,26-27,31,33,37,42-43H,2-20,22-23,25,28-29H2,1H3,(H,38,44)/b24-21+/t31-,33+/m0/s1
InChi Key
SNOJCYCOPNIGIK-ULETYAGTSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $197

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    5 mg

    $838

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    10 mg

    $1,576

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    Product Description

    C12 NBD ceramide (d18:1/12:0) is a long-chain fluorescent ceramide analog that can be used for the measurement of alkaline and neutral ceramidase activity.1 It is hydrolyzed efficiently by alkaline ceramidase from P. aeruginosa and by neutral ceramidase from mouse liver. In addition, alkaline and neutral ceramidases from a variety of mammalian cell lines also hydrolyze C12 NBD ceramide (d18:1/12:0). Acid ceramidase does not appear to utilize C-12 NBD ceramide as a substrate. The possibility exists for C12 NBD ceramide (d18:1/12:0) to also be applied to the measurement of ceramide kinase and/or sphingomyelin synthase activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Tani, M., Okino, N., Mitsutake, S., et al. Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. J. Biochem. 125(4), 746-749 (1999).

    Product Citations

    Otsuka, Y., Airola, M.V., Choi, Y.-M., et al. Identification of small-molecule inhibitors of neutral ceramidase (nCDase) via target-based high-throughput screening. SLAS Discov. (2020).