Nuclei counter stain for fluorescent immunocytochemical testing
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Propidium Iodide Solution

Item No. 10008351

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • PI Solution
Purity
≥95%
Formulation (Request formulation change)
A 1 mg/ml solution in PBS, pH 7.4, 5 ml per vial
SMILES
NC1=CC(C(C2=CC=CC=C2)=[N+](CCC[N+](CC)(C)CC)C3=CC(N)=CC=C34)=C4C=C1.[I-].[I-]
InChi Code
InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChi Key
XJMOSONTPMZWPB-UHFFFAOYSA-M
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    Product Description

    Propidium Iodide (PI) binds to double-stranded DNA and RNA after cells have been permeabilized. Once bound to nucleic acids, the nuclei appear red in color as observed by fluorescence microscopy with a rhodamine filter. PI is useful for multi-color fluorescent staining analysis. The bright red staining of nuclei makes this an excellent counter stain to be used simultaneously with FITC, PE, or Cy5. For analysis by flow cytometry, PI staining can be monitored in the FL2 channel. PI can also be used to measure cell viability since this dye is excluded from viable cells.

    WARNING This product is not for human or veterinary use.