For immunochemical detection of CREB
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CREB (Phospho-Ser133) Polyclonal Antibody

Item No. 10009181

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
Synthetic peptide from amino acid residues surrounding the phospho-Ser133 of rat CREB
MW
45 kDa
Formulation (Request formulation change)
100 µl of affinity-purified polyclonal antibody
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) Rat CREB
Species Reactivity
(+) Mouse(+) Rat
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    1 ea

    $464

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    Product Description

    It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus.1 The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors.2 This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory.3,4 A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 is required for CREB-mediated transcription.5,6

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Karin, M., and Hunter, T. Transcriptional control by protein phosphorylation: Signal transmission from the cell surface to the nucleus. Curr. Biol. 5(7), 747-757 (1995).

    2. Montminy, M. Transcriptional regulation by cyclic AMP. Annu. Rev. Biochem. 66, 807-822 (1997).

    3. Shaywitz, A.J., and Greenberg, M.E. CREB: A stimulus-induced transcription factor activated by a diverse array of extracellular signals. Annu. Rev. Biochem. 68, 821-861 (1999).

    4. Silva, A.J., Kogan, J.H., Frankland, P.W., et al. CREB and memory. Annu. Rev. Neurosci. 21, 127-148 (1998).

    5. Johannessen, M., Delghandi, M.P., and Moens, U. What turns CREB on? Cell. Signal. 16(11), 1211-1227 (2004).

    6. Kornhauser, J.M., Cowan, C.W., Shaywitz, A.J., et al. CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 34(2), 221-233 (2002).