For the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates
Features
  • Isolate nuclear and cytoplasmic fractions from cell lysates and tissue homogenates
  • Resulting isolates can be used in Transcription Factor Assay Kits
  • Isolates can also be used for EMSA or western blotting
  • Each kit provides reagents for 50 extractions from approximately 10 million cells
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Nuclear Extraction Kit

Item No. 10009277

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    $219

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    Product Description

    Preparation of nuclear extracts is the first step in examining transcription factor activity. Cayman’s Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in Cayman’s Transcription Factor Assay kits. The proteins isolated using this kit can also be used in electrophoretic mobility shift assays (EMSA) and western blotting applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

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    Kamarajugadda, S., Becker, J.R., Hanse, E.A., et al. Cyclin D1 represses peroxisome proliferator-activated receptor alpha and inhibits fatty acid oxidation. Oncotarget 7(30), 47674-47686 (2016).

    Zhang, H., Shao, Z., Alibin, C.P., et al. Liganded peroxisome proliferator-activated receptors (PPARs) preserve nuclear histone deacetylase 5 levels in endothelin-treated Sprague-Dawley rat cardiac myocytes. PLoS One 9(12), e115258 (2014).

    Cho, Y.H., Ku, C.R., Hong, Z.-Y., et al. Therapeutic effects of water soluble danshen extracts on therosclerosis. Evid. Based Complement. Alternat. Med. 2013, 623639 (2013).

    Li, H., Wang, Y., Zhang, H., et al. Yohimbine enhances protection of berberine against LPS-induced mouse lethality through multiple mechanisms. PLoS One 7(12), (2012).

    Thering, B.J., Bionaz, M., and Loor, J.J. Long-chain fatty acid effects on peroxisome proliferator-activated receptor-a-regulated genes in Madin-Darby bovine kidney cells: Optimization of culture conditions using palmitate. J. Dairy Sci. 92, 2027-2037 (2009).