LCAT Polyclonal Antibody

Lecithin:cholesterol acyltransferase (LCAT) has both phospholipase and acyltransferase activities. LCAT catalyzes the fatty acid transfer from the sn-2 position of phosphatidylcholine (lecithin) to cholesterol and to a lesser degree to other acceptor molecules.^1,2 This enzyme is critical to the process of reverse cholesterol transport or movement of cholesterol esters into high density lipoprotein (HDL) particles from cells.³ LCAT is abundant in blood-plasma, however it is present in other fluids and tissues such as ovary. This protein may be detected on immunoblot at 49.5 kDa as well as at 66 kDa when fully glycosylated.^4,5 Plasma samples should be diluted prior to electrophoresis and immunoblotting to avoid the band-broadening effect caused by comigration with endogenous albumin.

WARNING This product is not for human or veterinary use.

1. Glomset, J.A. Physiological role of lecithin cholesterol acyltransferase. Am. J. Clin. Nutr. 23(8), 1129-1136 (1970).

2. McLean, J., Fielding, C., Drayna, D., et al. Cloning and expression of human lecithin-cholesterol acyltransferase cDNA. Proc. Natl. Acad. Sci. USA 83(8), 2335-2339 (1986).

3. Linsel-Nitschke, P., and Tall, A.R. HDL as a target in the treatment of atherosclerotic cardiovascular disease. Nat. Rev. Drug Discov. 4(3), 193-205 (2005).

4. Marcel, Y.L., Vézina, C.A., Weech, P.K., et al. Lecithin: Cholesterol Acyltransferase, a review and immunochemical studies. Adv. Exp. Med. Biol. 201, 163-179 (1986).

5. Chisholm, J.W., Gebre, A.K., and Parks, J.S. Characterzation of C-terminal histidine-tagged human recombinant lecithin:cholesterol acyltransferase. J. Lipid Res. 40(8), 1512-1519 (1999).