A colorimetric 96-well assay for inorganic phosphate
Features
  • Measure inorganic free phosphate in aqueous solutions
  • Assay 42 samples in duplicate
  • Assay Range: 1.6-25 µM
  • Plate-based colorimetric measurement (620-640 nm)
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Malachite Green Phosphate Assay Kit

Item No. 10009325

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    2 x 96 wells

    $219

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    Product Description

    Cayman’s Malachite Green Phosphate Assay Kit provides a fast, reproducible, colorimetric method for measuring inorganic free phosphate in aqueous solutions. The assay method is based on the formation of a complex between malachite green molybdate and free orthophosphate that absorbs at 620-640 nm.1,2 Applications for this assay include quantification of phosphorylation and phosphate release from protein phosphatase substrates.3 This assay is a reliable and suitable means of detecting and quantifying minimal amounts of inorganic free phosphate and is amenable to high-throughput screening applications.4 The assay is formatted to a 96-well format, but could easily be modified for use in 384-well or cuvette-based assays.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. D'Angelo, E., Crutchfield, J., and Vandiviere, M. Rapid, sensitive, microscale determination of phosphate in water and soil. J. Environ. Qual. 30, 2206-2209 (2001).

    2. O'Toole, M., Lau, K.T., Shepherd, R., et al. Determination of phosphate using a highly sensitive paired emitter-detector diode photometric flow detector. Anal. Chim. Acta 597, 290-294 (2007).

    3. Maehama, T., Taylor, G.S., Slama, J.T., et al. A sensitive assay for phosphoinositide phosphatases. Anal. Biochem. 279, 248-250 (2000).

    4. Attin, T., Becker, K., Hannig, C., et al. Suitability of a malachite green procedure to detect minimal amounts of phosphate dissolved in acidic solutions. Clin. Oral Investig. 9(3), 203-207 (2005).

    Product Citations

    Balamurugan, K., Medishetti, R., Kotha, J., et al. PHLPP1 promotes neutral lipid accumulation through AMPK/ChREBP-dependent lipid uptake and fatty acid synthesis pathways. iScience 25, 103766 (2022).

    Wang, T., Liu, J., McDonald, C., et al. GDF15 is a heart-derived hormone that regulates body growth. EMBO Mol. Med. 9(8), 1150-1164 (2017).

    Cerezo, M., Lehraiki, A., Millet, A., et al. Compounds triggering ER stress exert anti-melanoma effects and overcome BRAF inhibitor resistance. Cancer Cell 29(6), 805-819 (2016).

    Biswas, N., Friese, R.S., Gayen, J.R., et al. Discovery of a novel target for the dysglycemic chromogranin A fragment pancreastatin: interaction with the chaperone GRP78 to influence metabolism. PLoS One 9(1), e84132 (2014).