For detection of viable cells
Features
  • A colorimetric, non-radioactive assay for assessing cell viability and proliferation
  • MTT is reduced to insoluble, purple formazan by NAD(P)H-oxidoreductases in living cells
  • Detect 10,000-250,000 cells per well
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MTT Cell Proliferation Assay Kit

Item No. 10009365

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    480 wells

    $219

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    Product Description

    Cayman’s MTT Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. This kit will also allow investigators to screen drug candidates involved in cell cycle regulation. In this assay, MTT is taken up by cells through the plasma membrane potential and then reduced to formazan by intracellular NAD(P)H-oxidoreductases.1

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol. Ann. Rev. 11, 127-152 (2005).

    Product Citations

    Kundu, D., Franchini, L., Hasdemir, H.S., et al. Distinct interactions of cannabinol and its cytochrome P450-generated metabolites with receptors and sensory neurons. J. Med. Chem. 68(13), 13935-13953 (2025).

    Roy, P., Maturano, J., Hasdemir, H., et al. Elucidating the mechanism of metabolism of cannabichromene by human cytochrome P450s. J. Nat. Prod. 87(4), 639-651 (2024).

    Dennis, D.G., Anand, S.D., Lopez, A.J., et al. Synthesis of the cannabimovone and cannabifuran class of minor phytocannabinoids and their anti-inflammatory activity. The Journal of Organic Chemistry 87(9), 6075-6086 (2022).

    Kotla, S., Sing, N.K., and Rao, G.N. ROS via BTK-p300-STAT1-PPARγ signaling activation mediates cholesterol crystals-induced CD36 expression and foam cell formation. Redox Biol. 11, 350-364 (2017).