Active • Host: Insect cells • AA: 656-1,122 • Tag: C-terminal His • MW: 51.0 kDa
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HDAC5 (human, recombinant)

Item No. 10009379

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Histone Deacetylase 5
Formula Weight
51.0
Purity
≥90%
Source
10 µg of active recombinant human C-terminal His-tagged HDAC5 catalytic domain expressed in insect cells
Amino Acids
656–1,122
MW
51 kDa
Formulation (Request formulation change)
45 mM Tris-HCl, pH 8.0, 124 mM sodium chloride, 2.4 mM potassium chloride, 225 imidazole, and 10% glycerol
UniProt Accession №
Q9UQL6
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    10 µg

    $533

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    Product Description

    Histone deacetylase 5 (HDAC) is a zinc-dependent metalloenzyme and class IIa HDAC.1 It is composed of an N-terminal regulatory domain, which contains a myocyte-specific enhancer factor 2 (MEF2) binding site, two 14-3-3 binding sites, and a nuclear localization signal, a catalytic domain, and a C-terminal domain that contains a nuclear export signal. HDAC5 shuttles between the cytoplasm and nucleus in a manner dependent on calcium/calmodulin-dependent protein kinase (CaMK) and 14-3-3 and is mainly expressed in the heart, but is also found in brain, breast, colon, and prostate tissues.2,3,4 It acts as a transcriptional corepressor and has many binding partners, including the transcriptional coactivator myocardin, which is involved in smooth muscle differentiation.1 Knockout of Hdac5 induces cardiac hypertrophy in mice and impairs spatial memory formation in a mouse model of Alzheimer’s disease.5,6 Tumor levels of HDAC5 are increased in various cancer types.7 Cayman’s HDAC5 (human, recombinant) protein can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Yang, X.J., and Grégoire, S. Class II histone deacetylases: From sequence to function, regulation, and clinical implication. Mol. Cell. Biol. 25(8), 2873-2884 (2005).

    2. McKinsey, T.A., Zhang, C.L., and Olson, E.N. Identification of a signal-responsive nuclear export sequence in class II histone deacetylases. Mol. Cell. Biol. 21(18), 6312-6321 (2001).

    3. Grozinger, C.M., and Schreiber, S.L. Regulation of histone deacetylase 4 and 5 and transcriptional activity by 14-3-3-dependent cellular localization. Proc. Natl. Acad. Sci. USA 97(14), 7835-7840 (2000).

    4. de Ruijter, A.J., van Gennip, A.H., Caron, H.N., et al. Histone deacetylases (HDACs): Characterization of the classical HDAC family. Biochem J. 370(Pt 3), 737-749 (2003).

    5. Chang, S., McKinsey, T.A., Zhang, C.L., et al. Histone deacetylases 5 and 9 govern responsiveness of the heart to a subset of stress signals and play redundant roles in heart development. Mol. Cell. Biol. 24(19), 8467-8476 (2004).

    6. Agis-Balboa, R.C., Pavelka, Z., Kerimoglu, C., et al. Loss of HDAC5 impairs memory function: Implications for Alzheimer’s disease. J. Alzheimers Dis. 33(1), 35-44 (2013).

    7. Yang, J., Gong, C., Ke, Q., et al. Insights into the function and clinical application of HDAC5 in cancer management. Front. Oncol. 11:661620, (2021).