For immunochemical detection of ATF2
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

ATF2 (Phospho-Ser490,498) Polyclonal Antibody

Item No. 10009410

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Activating Transcription Factor 2
Immunogen
synthetic phosphopeptide corresponding to amino acid residues surrounding phospho-Ser490,498 of human ATF2
Formulation (Request formulation change)
Peptide affinity-purified antibody
Host
Rabbit
Applications
WB and IHC (frozen sections)
Species Reactivity
(+) Human ATF2
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $464

    Add
    Product Description

    The activating transcription factor 2 (ATF2; also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins.1 ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control, and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2.2,3 Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF.4,3 Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered an important substrate of signals upstream of the activation of genes associated with neuronal growth and differentiation.5 ATF expression has also been linked to depression in humans.6

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Maekawa, T., Sakura, H., Kanei-Ishii, C., et al. Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain. EMBO J. 8, 2023-2028 (1989).

    2. Ivanov, V.N., Bhoumik, A., and Ronai, Z. Death receptors and melanoma resistance to apoptosis. Oncogene 22, 3152-3161 (2003).

    3. Morton, S., Davis, R.J., and Cohen, P. Signalling pathways involved in multisite phosphorylation of the transcription factor ATF2. FEBS Lett. 572, 177-183 (2004).

    4. Fuchs, S.Y., Tappin, I., and Ronai, Z. Stability of the ATF2 transcription factor is regulated by phosphorylation and dephosphorylation. The Journal of Biological Chemisty 275, 12560-12564 (2000).

    5. Karin, M., and Hunter, T. Transcriptional control by protein phosphorylation: Signal transmission from the cell surface to the nucleus. Curr. Biol. 5(7), 747-757 (1995).

    6. Laifenfeld, D., Karry, R., Grauer, E., et al. ATF2, a member of the CREB/ATF family of transcription factors, in chronic stress and consequent to antidepressant treatment: Animal models and human post-mortem brains. Neuropsychopharmacology 29, 589-597 (2004).