For immunochemical detection of tyrosine hydroxylase
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Tyrosine Hydroxylase (Phospho-Ser40) Polyclonal Antibody

Item No. 10009414

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • TH
Immunogen
Synthetic peptide from amino acid residues surrounding phospho-Ser40 of rat TH
MW
60 kDa
Formulation (Request formulation change)
100 µl of affinity-purified polyclonal antibody
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol
Host
Rabbit
Isotype
IgG
Applications
WB, IF, IHC
Species Reactivity
(+) Mammalian TH(+) Non-mammalian TH
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease, and drug abuse.1,2,3 TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling.4,5,6 The activity of TH is also regulated by phosphorylation.7,8,9 Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kish, S.J., Kalasinsky, K.S., Derkach, P., et al. Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24(5), 561-567 (2001).

    2. Zhu, M.-Y., Klimek, V., Haycock, J.W., et al. Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J. Neurosci. Methods 99(1-2), 37-44 (2000).

    3. Zhu, M.-Y., Klimek, V., Dilley, G.E., et al. Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol. Psychiatry 46(9), 1275-1286 (1999).

    4. Witkovsky, P., Gabriel, R., Haycock, J.W., et al. Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J. Chem. Neuroanat. 19(2), 105-116 (2000).

    5. Salvatore, M.F., Waymire, J.C., and Haycock, J.W. Depolarization-stimulated catecholamine biosynthesis: Involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J. Neurochem. 79(2), 349-360 (2001).

    6. Dunkley, P.R., Bobrovskaya, L., Graham, M.E., et al. Tyrosine hydroxylase phosphorylation: Regulation and consequences. J. Neurochem. 91(5), 1025-1043 (2004).

    7. Haycock, J.W., Ahn, N.G., Cobb, M.H., et al. ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ. Proc. Natl. Acad. Sci. USA 89(6), 2365-2369 (1992).

    8. Haycock, J.W., Bennett, W.F., George, R.J., et al. Multiple site phosphorylation of tyrosine hydroxylase. Differential regulation in situ by a 8-bromo-cAMP and acetylcholine. The Journal of Biological Chemisty 257(22), 13699-13703 (1982).

    9. Jedynak, J.P., Ali, S.F., Haycock, J.W., et al. Acute administration of cocaine regulates the phosphorylation of serine-19,-31 and -40 in tyrosine hydroxylase. J. Neurochem. 82(2), 382-388 (2002).