For immunochemical detection of Raf-1
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Raf-1 (Phospho-Ser642) Polyclonal Antibody

Item No. 10009505

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
phosphopeptide corresponding to amino acid residues surrounding phospho-Ser642 of rat raf-1
Formulation (Request formulation change)
Affinity-purified IgG
Host
Rabbit
Applications
WB
Species Reactivity
(+) Rat Raf-1(+) Bovine Raf-1 (expected)(+) Canine Raf-1 (expected)(+) Chicken Raf-1 (expected)(+) Human Raf-1 (expected)(+) Mouse Raf-1 (expected)(+) Non-human primate (expected)(+) Xenopus Raf-1 (expected)
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea

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    Product Description

    The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK.1 Previous studies have shown that phosphorylation is required for Raf-1 activation.2,3 Recent work has demonstrated that phosphorylation also regulates the down regulation of Raf with two sites participating: Ser301 and Ser642.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Marshall, C.J. Ras effectors. Curr. Opin. Cell Biol. 8, 197-204 (1996).

    2. Chong, H., Vikis, H.G., and Guan, K.L. Mechanisms of regulating the Raf kinase family. Cell. Signal. 15(5), 463-469 (2003).

    3. Dhillon, A.S., and Kolch, W. Untying the regulation of the Raf-1 kinase. Arch. Biochem. Biophys. 404, 3-9 (2002).

    4. Dougherty, M.K., Müller, J., Ritt, D.A., et al. Regulation of Raf-1 by direct feedback phosphorylation. Mol. Cell 17, 215-224 (2005).