Active • Host: E. coli • AA: 1-132 (full length) • Tag: N-terminal His • MW: 18.8 kDa
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FABP4 (human, recombinant)

Item No. 10009549

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Adipocyte FABP
  • A-FABP
  • ALBP
  • aP2
  • Fatty Acid Binding Protein 4
  • Fatty Acid Binding Proetein (Adipocyte)
Purity
≥90% estimated by SDS-PAGE
Source
Active N-terminal His-tagged recombinant human FABP4 expressed in E. coli
Amino Acids
1-132 (full length)
MW
18.8 kDa
Formulation (Request formulation change)
50 mM of sodium phosphate, pH 7.2, with 100 mM sodium chloride and 20% glycerol
UniProt Accession №
P15090
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $388

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    Product Description

    Fatty acid binding protein 4 (FABP4) is a member of the intracellular lipid-binding protein (iLBP) family.1 It is composed of a β-barrel, which forms the lipid-binding site, and two α-helices that form a lid on the β-barrel.2 FABP4 is primarily expressed in adipocytes but has also been found in activated macrophages.3,4 It is involved in lipid transport and metabolism, insulin resistance, and angiogenesis, and its expression is induced by long-chain fatty acids and peroxisome proliferator-activated receptor γ (PPARγ) agonists.1,5,6,7,8 Knockout of Fabp4 prevents the development of atherosclerosis in ApoE-/- mice and increases body weight gain and insulin sensitivity in ob/ob mice.5,9 Serum levels of FABP4 are increased in patients with colorectal cancer, and serum and synovial fluid levels of FABP4 are increased in patients with rheumatoid arthritis.7,10 Cayman’s FABP4 (human, recombinant) protein can be used for lipid-binding assays and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zimmerman, A.W., and Veerkamp, J.H. New insights into the structure and function of fatty acid-binding proteins. Cell. Mol. Life Sci. 59(7), 1096-1116 (2002).

    2. Reese-Wagoner, A., Thompson, J., and Banaszak, L. Structural properties of the adipocyte lipid binding protein. Biochim. Biophys. Acta 1441(2-3), 106-116 (1999).

    3. Baxa, C.A., Sha, R.S., Buelt, M.K., et al. Human adipocyte lipid-binding protein: Purification of the protein and cloning of its complementary DNA. Biochemistry 28(22), 8683-8690 (1989).

    4. Perrella, M.A., Pellacani, A., Layne, M.D., et al. Absence of adipocyte fatty acid binding protein prevents the development of accelerated atherosclerosis in hypercholesterolemic mice. The FASEB Journal 15(10), 1774-1776 (2001).

    5. Uysal, K.T., Scheja, L., Wiesbrock, S.M., et al. Improved glucose and lipid metabolism in genetically obese mice lacking aP2. Endocrinology 141(9), 3388-3396 (2000).

    6. Hotamisligil, G.S., Johnson, R.S., Distel, R.J., et al. Uncoupling of obesity from insulin resistance through a targeted mutation in aP2, the adipocyte fatty acid binding protein. Science 274(5291), 1377-1379 (1996).

    7. Guo, D., Lin, C., Lu, Y., et al. FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis. Bone Res. 10(1), 45 (2022).

    8. Bastie, C., Holst, D., Gaillard, D., et al. Expression of preoxisome proliferator-activated receptor PPARδ promotes induction of PPARγ and adipocyte differentiation in 3T3C2 fibroblasts. The Journal of Biological Chemisty 274(31), 21920-21925 (1999).

    9. Lim, G.P., Yang, F., Chu, T., et al. Ibuprofen suppresses plaque pathology and inflammation in a mouse model for Alzheimer’s disease. J. Neurosci. 20(15), 5709-5714 (2000).

    10. Zhang, Y., Zhao, X., Deng, L., et al. High expression of FABP4 and FABP6 in patients with colorectal cancer. World J. Surg. Oncol. 17(1), 171 (2019).