Active • Host: Insect cells • AA: 627-1,085 • Tag: N-terminal GST • MW: 75.2 kDa
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HDAC4 (human, recombinant)

Item No. 10009652

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Histone Deacetylase 4
Purity
≥60% estimated by SDS-PAGE
Source
Active recombinant human N-terminal GST-tagged HDAC4 catalytic domain expressed in insect cells
Amino Acids
627-1,085
MW
75.2 kDa
Formulation (Request formulation change)
45 mM Tris-HCl, pH 8.0, with 124 mM sodium chloride, 2.4 mM potassium chloride, 18 mM glutathione, and 10% glycerol
UniProt Accession №
P56524
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    10 µg

    $557

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    Cayman Chemical
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    Product Description

    Histone deacetylase 4 (HDAC4) is a zinc-dependent metalloenzyme and class IIa HDAC.1 It is composed of an N-terminal regulatory domain, which contains a myocyte-specific enhancer factor 2 (MEF2) binding site, three 14-3-3 binding sites, a nuclear localization signal, and a caspase-3 cleavage site, a catalytic domain, and a C-terminal domain that contains a nuclear export signal. HDAC4 shuttles between the cytoplasm and nucleus and is mainly expressed in skeletal muscle, brain, ovaries, and colon but is also found in the small intestine, heart, kidney, testis, thymus, and leukocytes.2,3 It acts as a transcriptional corepressor and has many binding partners, including the transcription factors MEF2 and RUNX family transcription factor 2 (RUNX2).1,4 Knockout of Hdac4 induces premature ossification and chondrocyte hypertrophy in mice.4 Mutations in HDAC4 are associated with brachydactyly-mental retardation syndrome, a disorder characterized by brachydactyly type E, intellectual disability, and facial dysmorphisms.5 Cayman’s HDAC4 (human, recombinant) protein can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Yang, X.J., and Grégoire, S. Class II histone deacetylases: From sequence to function, regulation, and clinical implication. Mol. Cell. Biol. 25(8), 2873-2884 (2005).

    2. Wang, A.H., Kruhlak, M.J., Wu, J., et al. Regulation of histone deacetylase 4 by binding of 14-3-3 proteins. Mol. Cell. Biol. 20(18), 6904-6912 (2000).

    3. Wang, A.H., Bertos, N.R., Vezmar, M., et al. HDAC4, a human histone deacetylase related to yeast HDA1, is a transcriptional corepressor. Mol. Cell. Biol. 19(11), 7816-7827 (1999).

    4. Vega, R.B., Matsuda, K., Oh, J., et al. Histone deacetylase 4 controls chondrocyte hypertrophy during skeletogenesis. Cell 119(4), 555-566 (2004).

    5. Wakeling, E., McEntagart, M., Bruccoleri, M., et al. Missense substitutions at a conserved 14-3-3 binding site in HDAC4 cause a novel intellectual disability syndrome. HGG Adv. 2(1), 100015 (2021).