For immunochemical detection of the NMDA Receptor
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NMDA Receptor NR2B Subunit (Phospho-Tyr1472) Polyclonal Antibody

Item No. 10009761

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
phosphopeptide corresponding to amino acid residues surrounding the Phospho-Tyr1472 of NMDA Receptor NR2B Subunit
Formulation (Request formulation change)
Peptide affinity-purified antibody
Host
Rabbit
Applications
WB
Species Reactivity
(+) Human NMDA receptor(+) Rat NMDA receptor(+) Bovine NMDA receptor (expected)(+) Canine NMDA receptor (expected)(+) Chicken NMDA receptor (expected)(+) Mouse NMDA receptor (expected)(+) Non-human primates NMDA receptor (expected)(+) Zebrafish NMDA receptor (expected)
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s disease, epilepsy and ischemic neuronal death. 1,2,3 The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS. 4,5,6 Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. 7 Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. 8,9 Recent work suggests that phosphorylation of Tyr1472 on NR2B may regulate the functional expression of the receptor in LTP and other forms of plasticity. 10,11

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Grosshans, D.R., Clayton, D.A., Coultrap, S.J., et al. LTP leads to rapid surface expression of NMDA but not AMA receptors in adult rat CA1. Nat. Neurosci. 5, 27-33 (2002).

    2. Wenthold, R.J., Prybylowski, K., Standley, S., et al. Trafficking of NMDA receptors. Annu. Rev. Pharmacol. Toxicol. 43, 335-358 (2003).

    3. Carroll, R.C., and Zukin, R.S. NMDA-receptor trafficking and targeting: Implications for synaptic transmission and plasticity. Trends Neurosci. 25(11), 571-577 (2002).

    4. Lovinger, D.M., White, G., and Weight, F.F. Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243, 1721-1724 (1989).

    5. Alvestad, R.M., Grosshans, D.R., Coultrap, S.J., et al. Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. The Journal of Biological Chemisty 278(13), 11020-11025 (2003).

    6. Snell, L.D., Nunley, K.R., Lickteig, R.L., et al. Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion. Brain Res. Mol. Brain Res. 40, 71-78 (1996).

    7. Ishii, T., Moriyoshi, K., Sugihara, H., et al. Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits. The Journal of Biological Chemisty 268(4), 2836-2843 (1993).

    8. Clayton, D.A., Grosshans, D.R., and Browning, M.D. Aging and surface expression of hippocampal NMDA receptors. The Journal of Biological Chemisty 277(17), 14367-14369 (2002).

    9. Clayton, D.A., Mesches, M.H., Alvarez, E., et al. A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat. J. Neurosci. 22, 3628-3637 (2002).

    10. Nakazawa, T., Komai, S., Tezuka, T., et al. Characterization of Fyn-mediated tyrosine phosphorylation sites on GluR.epislon.2 (NR2B) subunit of the N-methyl-D-aspartate receptor. The Journal of Biological Chemisty 276(1), 693-699 (2001).

    11. Roche, K.W., Standley, S., McCallum, J., et al. Molecular determinants of NMDA receptor internalization. Nat. Neurosci. 4, 794-802 (2001).

    Product Citations

    Giralt, A., Brito, V., Chevy, Q., et al. Pyk2 modulates hippocampal excitatory synapses and contributes to cognitive deficits in a Huntington’s disease model. Nat. Commun. 8, 15592 (2017).