PIP2 Binding Protein
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PtdIns-(4,5)-P2 Binding Protein (GST-tagged)

Item No. 10009815

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Phosphatidylinositol-4,5-diphosphate
  • PI(4,5)-P2
  • PI(4,5)P2 Binding Protein
  • PIP2
  • PIP2[4',5'] Binding Protein
  • PLC-δ1-PH Domain
Purity
≥90%
Source
Recombinant GST-tagged protein purified from E. coli
MW
~45.2 kDa
Formulation (Request formulation change)
A solution in PBS, pH 7.4, containing 20% glycerol
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    25 µg

    $134

    Add

    50 µg

    $253

    Add

    100 µg

    $477

    Add
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    Product Description

    Pleckstrin homology (PH) domains contain a seven-standard β-sandwich that allows binding to various species of phosphatidylinositol (PtdIns) phosphates.1 PtdIns-(4,5)-P2 binding protein contains a highly specific PH domain that recognizes and binds PtdIns-(4,5)-P2 in the plasma membrane.2,3 PtdIns-(4,5)-P2 can be phosphorylated by PI3K to make PtdIns-(3,4,5)-P3, which initiates an intricate signaling cascade that has been implicated in cancer.4 Cayman's PtdIns-(4,5)-P2 Binding Protein (GST-tagged) can be used in vitro for the detection of PtdIns-(4,5)-P2 in PI3K and PTEN activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lemmon, M.A., and Ferguson, K.M. Molecular determinants in pleckstrin homology domains that allow specific recognition of phosphoinositides. Biochem. Soc. Trans. 29(4), 377-384 (2001).

    2. Lemmon, M.A. Phosphoinositide recognition domains. Traffic 4(4), 201-213 (2003).

    3. Garcia, P., Gupta, R., Shah, S., et al. The pleckstrin homology domain of phospholipase C-δ1 binds with high affinity to phosphatidylinositol 4,5-bisphosphate in bilayer membranes. Biochemistry 34(49), 16228-16234 (1995).

    4. Vivanco, I., and Sawyers, C.L. The phosphatidylinositol 3-kinase-AKT pathway in human cancer. Nat. Rev. Cancer 2(7), 489-501 (2002).