PIP2 Binding Protein
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PtdIns-(4,5)-P2 Binding Protein (GST-tagged)

Item No. 10009815

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Phosphatidylinositol-4,5-diphosphate
  • PI(4,5)-P2
  • PI(4,5)P2 Binding Protein
  • PIP2
  • PIP2[4',5'] Binding Protein
  • PLC-δ1-PH Domain
Purity
≥90%
Source
Recombinant GST-tagged protein purified from E. coli
MW
~45.2 kDa
Formulation (Request formulation change)
A solution in PBS, pH 7.4, containing 20% glycerol
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    Product Description

    Pleckstrin homology (PH) domains all contain a seven-standard b-sandwich that allows binding to various species of phosphatidylinositol (PtdIns) phosphates.1 Most PH domains bind to PtdIns phosphates with weak affinity and low specificity. However, a small subclass binds specifically and with high-affinity for certain PtdIns phosphates. PtdIns-(4,5)-P2 Binding Protein (GST-tagged) contains a highly specific PH domain that recognizes and binds PtdIns-(4,5)-P2 (see Figure 1).2 PtdIns phosphates represent a small percentage of total membrane phospholipids. However, they play a critical role in the generation and transmission of cellular signals.3,4 PtdIns-(4,5)-P2 can be phosphorylated by phosphoinositide (PI)-3-kinase to make PtdIns-(3,4,5)-P3 which initiates an intricate signaling cascade that has been implicated in cancer5 PtdIns-(4,5)-P2 binding protein can be used in in vitro assays for the detection of PtdIns-(4,5)-P2 in PI3-kinase and PTEN phosphatase assays.

    WARNING This product is not for human or veterinary use.