PIP2 Binding Protein
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PtdIns-(4,5)-P2 Binding Protein (GST-tagged)

Item No. 10009815

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Phosphatidylinositol-4,5-diphosphate
  • PI(4,5)-P2
  • PI(4,5)P2 Binding Protein
  • PIP2
  • PIP2[4',5'] Binding Protein
  • PLC-δ1-PH Domain
Purity
≥90%
Source
Recombinant GST-tagged protein purified from E. coli
MW
~45.2 kDa
Formulation (Request formulation change)
A solution in PBS, pH 7.4, containing 20% glycerol
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    25 µg

    $134

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    50 µg

    $253

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    100 µg

    $477

    Add
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    Product Description

    Pleckstrin homology (PH) domains all contain a seven-standard b-sandwich that allows binding to various species of phosphatidylinositol (PtdIns) phosphates.1 Most PH domains bind to PtdIns phosphates with weak affinity and low specificity. However, a small subclass binds specifically and with high-affinity for certain PtdIns phosphates. PtdIns-(4,5)-P2 Binding Protein (GST-tagged) contains a highly specific PH domain that recognizes and binds PtdIns-(4,5)-P2 (see Figure 1).2 PtdIns phosphates represent a small percentage of total membrane phospholipids. However, they play a critical role in the generation and transmission of cellular signals.3,4 PtdIns-(4,5)-P2 can be phosphorylated by phosphoinositide (PI)-3-kinase to make PtdIns-(3,4,5)-P3 which initiates an intricate signaling cascade that has been implicated in cancer5 PtdIns-(4,5)-P2 binding protein can be used in in vitro assays for the detection of PtdIns-(4,5)-P2 in PI3-kinase and PTEN phosphatase assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lemmon, M.A., and Ferguson, K.M. Molecular determinants in pleckstrin homology domains that allow specific recognition of phosphoinositides. Biochem. Soc. Trans. 29(4), 377-384 (2001).

    2. Lemmon, M.A. Phosphoinositide recognition domains. Traffic 4(4), 201-213 (2003).

    3. Exton, J.H. Regulation of phosphoinositide phospholipases by hormones, neurotransmitters, and other agonists linked to G proteins. Annu. Rev. Pharmacol. Toxicol. 36, 481-509 (1996).

    4. Vivanco, I., and Sawyers, C.L. The phosphatidylinositol 3-kinase-AKT pathway in human cancer. Nat. Rev. Cancer 2(7), 489-501 (2002).