PIP3 Binding Protein
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Product Categories

Product Type

Proteins

Research Area

Cancer
Cell Biology
Inositol Phosphates

Application

Western Blot

PtdIns-(3,4,5)-P3 Binding Protein

Item No. 10009817

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cyth3
  • Cytohesin 3
  • GRP1
  • GRP1 PH Domain
  • PIP3
  • PIP3[3',4',5'] Binding Protein
  • PI(3,4,5)-P3
  • PI(3,4,5)P3 Binding Protein
  • Phosphatidylinositol-3,4,5-triphosphate
Purity
≥95%
Source
Recombinant human N-terminal GST-tagged PIP3 expressed in E. coli
Amino Acids
264-381 (PH domain)
MW
~41.7 kDa
Formulation (Request formulation change)
A solution in PBS, pH 7.4, containing 20% glycerol
UniProt Accession №
O43739
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    25 µg

    $156

    Add

    50 µg

    $279

    Add

    100 µg

    $524

    Add
    Cayman Chemical
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    Product Description

    Pleckstrin homology (PH) domains all contain a seven-standard β-sandwich that allows binding to various species of phosphatidyl inositol (PtdIns) phosphates.1 Most PH domains bind to PtdIns phosphates with weak affinity and low specificity. However, a small subclass binds specifically and with high-affinity for certain PtdIns phosphates. PtdIns-(3,4,5)-P3 Binding Protein contains a highly specific PH domain that recognizes and binds PtdIns-(3,4,5)-P3 (see Figure 1).2 PtdIns phosphates represent a small percentage of total membrane phospholipids. However, they play a critical role in the generation and transmission of cellular signals.3,4 PtdIns-(4,5)-P2 can be phosphorylated by phosphoinositide (PI)-3-kinase to make PtdIns-(3,4,5)-P3 which initiates an intricate signalling cascade that has been implicated in cancer.5 Due to PtdIns-(3,4,5)-P3 binding protein · s unique affinity for PtdIns-(3,4,5)-P3, this protein can be used to detect product formed by PI3-kinase in in vitro assays. Cayman’s PtdIns-(3,4,5)-P3 Binding Protein is isoform 2, which differs from isoform 1 by the absence of a glycine residue at position 277.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lemmon, M.A., and Ferguson, K.M. Molecular determinants in pleckstrin homology domains that allow specific recognition of phosphoinositides. Biochem. Soc. Trans. 29(4), 377-384 (2001).

    2. Lemmon, M.A. Phosphoinositide recognition domains. Traffic 4(4), 201-213 (2003).

    3. Exton, J.H. Regulation of phosphoinositide phospholipases by hormones, neurotransmitters, and other agonists linked to G proteins. Annu. Rev. Pharmacol. Toxicol. 36, 481-509 (1996).

    4. Vivanco, I., and Sawyers, C.L. The phosphatidylinositol 3-kinase-AKT pathway in human cancer. Nat. Rev. Cancer 2(7), 489-501 (2002).