Assay for the quantification of phosphatidylcholine in plasma or serum
Features
  • Measure PC in serum or plasma
  • Assay 41 samples in duplicate
  • Plate-based colorimetric measurement (580-600 nm)
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Research Area

Phosphatidylcholine Colorimetric Assay Kit

Item No. 10009926

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • PC
Limit of Detection
0.212 mg/dl
Lower Limit of Quantification
2.5 mg/dl
Origin
Plant/Armoracia rusticana
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $385

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    Product Description

    Cayman’s phosphatidylcholine (PC) assay kit provides a specific, sensitive, and convenient method for quantifying PC in plasma or serum. In this assay, PC-Specific PLD is first used to hydrolyze PC to choline and phosphatidic acid. The newly formed choline is then used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-aminoantipyrine to generate a blue dye with an optimal absorption at 595 nm.1

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hojjati, M.R., and Jiang, X.C. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J. Lipid Res. 47(3), 673-676 (2006).

    Product Citations

    Yamamoto, Y., Yoshida, A., Miyazaki, N., et al. Arl6IP1 has the ability to shape the mammalian ER membrane in a reticulon-like fashion. Biochem. J. 458, 69-79 (2014).

    Zhou, Z., Bulgari, O., Vailati-riboni, M., et al. Rumen-protected methionine compared with rumen-protected choline improves immunometabolic status in dairy cows during the peripartal period. J. Dairy Sci. 99(11), 8956-8969 (2016).