Assay for the quantification of sphingomyelin in plasma or serum
Features
  • Measure SM in plasma and serum
  • Assay 41 samples in duplicate
  • Assay range: 5-50 mg/dl
  • Plate-based colorimetric measurement (585-600 nm)
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Sphingomyelin Colorimetric Assay Kit

Item No. 10009928

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • SM
Origin
Animal/Bovine, Plant/Armoracia rusticana
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $413

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    Product Description

    Cayman’s Sphingomyelin (SM) Assay Kit provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum. In this assay, sphingomyelinase is first used to hydrolyze SM to phosphorylcholine and ceramide. Alkaline phosphatase then generates choline from the phosphorylcholine and the newly formed choline is used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-aminoantipyrine to generate a blue color with an optimal absorption at 595 nm.1

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hojjati, M.R., and Jiang, X.C. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J. Lipid Res. 47(3), 673-676 (2006).

    Product Citations

    Estrach, S., Boulter, E., Cailleteau, L., et al. Hair Follicle Stem Cell SLC3A2 Regulates Epithelial Regenerative Properties. J. Invest. Dermatol. (2025).

    Tang, H.-B., Lu, Z.-L., Wei, X.-K., et al. Viperin inhibits rabies virus replication via reduced cholesterol and sphingomyelin and is regulated upstream by TLR4. Sci. Rep. 6, 30529 (2016).