A fluorescent probe for lipid-binding proteins
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1,8-ANS

Item No. 10010219

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
8-(phenylamino)-1-naphthalenesulfonic acid
CAS Number
82-76-8
Synonyms
  • 1-Anilinonaphthalene-8-Sulfonic Acid
Molecular Formula
C16H13NO3S
Formula Weight
299.3
Purity
≥95%
Emission
520 (free), 477 (bound)
Excitation
350 (free), 468 (bound)
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 20 mg/mlPBS (pH 7.2): 1 mg/ml
λmax
219, 270, 374 nm
SMILES
OS(=O)(=O)c1cccc2cccc(Nc3ccccc3)c12
InChi Code
InChI=1S/C16H13NO3S/c18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13/h1-11,17H,(H,18,19,20)
InChi Key
FWEOQOXTVHGIFQ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 g

    $36

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    25 g

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    50 g

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    Product Description

    1,8-ANS is a fluorescent dye that binds with high affinity to hydrophobic surfaces of proteins.1,2 1,8-ANS has an excitation maximum of 350 nm. It has an emission maximum of 520 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FABP2) it has emission maxima of 468-477 nm. 1,8-ANS binds to low polarity regions of protein surfaces making it well suited for determining the affinity of hydrophobic ligands to their corresponding binding proteins, such as the binding of free fatty acids to FABPs.1,2,3 1,8-ANS binds to FABP2 with a Kd value of approximately 9.7 µM at 24.5°C.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Pastukhov, A.V., and Ropson, I.J. Fluorescent dyes as probes to study lipid-binding proteins. Proteins 53(3), 607-615 (2003).

    2. Kirk, W.R., Kurian, E., and Prendergast, F.G. Characterization of the sources of protein-ligand affinity: 1-sulfonato-8-(1')anilinonaphthalene binding to intestinal fatty acid binding protein. Biophys. J. 70(1), 69-83 (1996).

    3. Ory, J.J., and Banaszak, L.J. Studies of the ligand binding reaction of adipocyte lipid binding protein using the fluorescent probe 1,8-anilinonaphthalene-8-sulfonate. Biophys. J. 77(2), 1107-1116 (1999).