FABP4 Inhibitor/Ligand Screening Assay Kit

Fatty acid binding protein 4 (Adipocyte-FABP, aP2, FABP4) is one of nine known cytosolic fatty acid binding proteins ranging in size from 14-15 kDa containing 127-132 amino acids.¹ FABP4 is highly expressed in adipocytes and is regulated by peroxisome-proliferator-activated receptor-γ (PPARγ) agonists, insulin and fatty acids. Recent studies using FABP4 gene deletion in mice indicate a dominant role for FABP4 in several chronic metabolic diseases. Therefore, inhibiting the function of FABP4 is a potential mechanism for the treatment of metabolic diseases like diabetes and atherosclerosis.^2,3 Cayman’s FABP4 Ligand Binding Assay Kit provides a simple, reproducible, and sensitive tool for the identification of FABP4 ligands. The assay makes use of a Detection Reagent that exhibits increased fluorescence at 500 nm when bound to FABP4. Any strong ligand and/or inhibitor of FABP4 will displace the Detection Reagent thereby reducing the fluorescence. FABP4 is provided in high purity and in sufficient quantity for 100 tests.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

1. Zimmerman, A.W., and Veerkamp, J.H. New insights into the structure and function of fatty acid-binding proteins. Cell. Mol. Life Sci. 59(7), 1096-1116 (2002).

2. Furuhashi, M., Tuncman, G., Görgün, C.Z., et al. Treatment of diabetes and atherosclerosis by inhibiting fatty-acid-binding protein aP2. Nature 447, 959-965 (2007).

3. Boord, J.B., Maeda, K., Makowski, L., et al. Adipocyte fatty acid-binding protein, aP2, alters late atherosclerotic lesion formation in severe hypercholesterolemia. Arterioscler. Thromb. Vasc. Biol. 22, 1686-1691 (2002).