For fluorescent analysis of SIRT1
Features
  • Screen for modulators of SIRT1 activity
  • Assay 45 samples in duplicate
  • Plate-based fluorometric measurement (ex 340-360 nm, em 450-465 nm)
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SIRT1 Direct Fluorescent Screening Assay Kit

Item No. 10010401

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Emission
450-465 nm
Excitation
350-360 nm
License
Sold under license from Cyclex.
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $383

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    Product Description

    Cayman’s SIRT1 Direct Screening Assay Kit provides a convenient fluorimetric method for screening SIRT1 modulators in a two-step reaction. In the first step, the SIRT1 peptide and NAD+ are incubated with human recombinant SIRT1. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product. The fluorophore can be easily analyzed using an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Damonte, P., Sociali, G., Parenti, M.D., et al. SIRT6 inhibitors with salicylate-like structure show immunosuppressive and chemosensitizing effects. Bioorg. Med. Chem. 25(20), 5849-5858 (2017).

    Aragonès, G., Suárez, M., Ardid-Ruiz, A., et al. Dietary proanthocyanidins boost hepatic NAD+ metabolism and SIRT1 expression and activity in a dose-dependent manner in healthy rats. Sci. Rep. 6, 24977 (2016).