10-Acetyl-3,7-dihydroxyphenoxazine (CAS 119171-73-2) | Cayman Chemical
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10-Acetyl-3,7-dihydroxyphenoxazine

Item № 10010469
CAS № 119171-73-2
Purity ≥98%
product image
                (CAS 119171-73-2)
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     1 mg $11.00 0.00
     5 mg $50.00 0.00
     10 mg $88.00 0.00
     25 mg $193.00 0.00

Pricing updated 2018-11-16. Prices are subject to change without notice.

Description
Synonyms
  • A 6550
  • ADHP
  • Amplex Red®

10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) is a highly sensitive, stable substrate for horseradish peroxidase (HRP) that enables selective detection of H2O2.1 This colorless, non-fluorescent reagent reacts with H2O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, ADHP enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl sample.1

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Technical Information
Formal Name
1-(3,7-dihydroxy-10H-phenoxazin-10-yl)-ethanone
CAS Number
119171-73-2
Synonyms
  • A 6550
  • ADHP
  • Amplex Red®
Molecular Formula
C14H11NO4
Formula Weight
257.2
Purity
≥98%
Formulation
A crystalline solid
λmax
250, 285 nm
SMILES
Oc1ccc2c(c1)Oc1cc(O)ccc1N2C(=O)C
InChI Code
InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3
InChI Key
PKYCWFICOKSIHZ-UHFFFAOYSA-N

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Stability
≥ 2 years
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Additional Information

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References & Product Citations
Product Description References

1. Zhou, M., Diwu, Z., Panchuk-Voloshina, N., et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: Applications in detecting the activity of phagocyte NADPH oxidase and other oxidases Analytical Biochemistry 253, 162-168 (1997).

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