A stable substrate for peroxidase detection
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ADHP

Item No. 10010469

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
1-(3,7-dihydroxy-10H-phenoxazin-10-yl)-ethanone
CAS Number
119171-73-2
Synonyms
  • A 6550
  • 10-Acetyl-3,7-dihydroxyphenoxazine
  • Amplex Red®
Molecular Formula
C14H11NO4
Formula Weight
257.2
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 25 mg/mlDMSO: 25 mg/mlDMSO:PBS (pH 7.2)1:5: 0.15 mg/mlEthanol: 1.5 mg/ml
λmax
250, 285 nm
SMILES
Oc1ccc2c(c1)Oc1cc(O)ccc1N2C(=O)C
InChi Code
InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3
InChi Key
PKYCWFICOKSIHZ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $28

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    5 mg

    $93

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    10 mg

    $143

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    25 mg

    $289

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    Product Description

    ADHP is a highly sensitive, stable substrate for horseradish peroxidase (HRP) that enables selective detection of H2O2.1 This colorless, non-fluorescent reagent reacts with H2O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, ADHP enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl sample.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zhou, M., Diwu, Z., Panchuk-Voloshina, N., et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: Applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal. Biochem. 253(2), 162-168 (1997).

    Product Citations

    Coussens, N.P., Kales, S.C., Henderson, M.J., et al. High-throughput screening with nucleosome substrate identifies small-molecule inhibitors of the human histone lysine methyltransferase NSD2. The Journal of Biological Chemisty 293(35), 13750-13765 (2018).