LDL Uptake Cell-Based Assay Kit

Low-density lipoproteins (LDL) is the major carrier of cholesterol in the blood, accounting for more than 60% of total plasma cholesterol. LDL is taken up by hepatic and extrahepatic tissues through LDL receptor-mediated endocytosis where it is internalized and degraded to free cholesterol and amino acids. Uptake of native and oxidized LDL by monocyte-derived macrophages in the vessel wall results in accumulation of cholesterol and formation of atherosclerotic plaques. The mechanism underlying the uptake of LDL by both hepatic tissue and cells of the cardiovascular system is not clearly understood. Thus, LDL uptake and its regulation are important therapeutic targets for atherosclerosis and related diseases. Cayman’s LDL Uptake Cell-Based Assay Kit provides a convenient tool for studying LDL uptake and regulation at cellular level. The kit employs human LDL conjugated to DyLight™ 550 as a fluorescent probe for detection of LDL uptake into cultured cells. A LDL receptor-specific polyclonal antibody and a DyLight™ 488-conjugated secondary antibody are included for identifying the distribution of LDL receptors.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

Cashikar, A.G., Toral-Rios, D., Timm, D., et al. Regulation of astrocyte lipid metabolism and ApoE secretionby the microglial oxysterol, 25-hydroxycholesterol. J. Lipid Res. 64(4), 100350 (2023).