HDAC Fluorometric Activity Assay Kit

Cayman’s HDAC Fluorometric Activity Assay Kit provides a fast, fluorometric method for measuring HDAC activity without the use of radioactivity, extraction, or chromatography. In the first step, an acetylated lysine substrate is incubated with samples. Deacetylation sensitizes the substrate such that treatment with the HDAC developer in the second step releases a fluorescent product. Trichostatin A is included as a control inhibitor. The fluorophore can be easily analyzed using a fluorescence plate reader with an excitation wavelength of 340-360 nm and an emission wavelength of 440-465 nm. The assay can be used for quantifying Class I and II HDAC activity from various sources.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

Witt, A.E., Lee, C.-W., Lee, T.I., et al. Identification of a cancer stem cell-specific function for the histone deacetylases, HDAC1 and HDAC7, in breast and ovarian cancer. Oncogene 36(12), 1707-1720 (2017).

Nehra, S., Bhardwaj, V., Ganju, L., et al. Nanocurcumin prevents hypoxia induced stress in primary human ventricular cardiomyocytes by maintaining mitochondrial homeostasis. PLoS One 10(9), e0139121 (2015).