For fluorescent analysis of SIRT3
Features
  • Screen for modulators of SIRT3 activity
  • Assay 45 samples in duplicate
  • Plate-based fluorometric measurement (ex 340-360 nm, em 450-465 nm)
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SIRT3 Direct Fluorescent Screening Assay Kit

Item No. 10011566

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Mitochondrial Nicotinamide Adenine Dinuclear-dependent Deacetylase
  • NAD-dependent Deacetylase
  • Silent Information Regulator 3
  • SIR2L3
  • SIR2-like Protein 3
  • ​Sirtuin 3
Emission
465 nm
Excitation
360 nm
License
Sold under license from Cyclex.
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    96 wells

    $383

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    Product Description

    Cayman’s SIRT3 Direct Fluorescent Screening Assay Kit provides a convenient fluorometric method for screening SIRT3 modulators in a two-step reaction. In the first step, the SIRT2/SIRT3 peptide and NAD+ are incubated with human recombinant SIRT3. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product. The fluorophore can be easily analyzed using an excitation wavelength of 360 nm and an emission wavelength of 465 nm.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Yoon, S.P., and Kim, J. Poly(ADP-ribose) polymerase 1 contributes to oxidative stress through downregulation of sirtuin 3 during cisplatin nephrotoxicity. Anat. Cell Biol. 49, 165-176 (2016).