A convenient fluorometric method of screening human sEH inhibitors
Features
  • Screen for inhibitors of human sEH in 40 minutes
  • Assay 44 samples in duplicate
  • Plate-based fluorometric measurement (Ex 330 nm, Em 465 nm)
  • Assay plate included
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Soluble Epoxide Hydrolase Inhibitor Screening Assay Kit

Item No. 10011671

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cytosolic Epoxide Hydrolase (CEH)
  • EPHX2
  • Epoxide Hydrolase 2
  • sEH
Emission
465 nm
Excitation
330 nm
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 wells

    $303

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    Product Description

    Cayman’s fluorescence-based sEH Inhibitor Screening Assay Kit provides a convenient method for screening human sEH inhibitors. The assay utilizes (3-phenyl-oxiranyl)-acetic acid cyano-(6-methoxy-naphthalen-2-yl)-methyl ester (PHOME) as a substrate. When the epoxide moiety of PHOME is hydrolyzed by sEH, an intramolecular cyclization occurs, which results in the release of a cyanohydrin under basic conditions. The cyanohydrin quickly decomposes into cyanide ion and the highly fluorescent 6-methoxy-2-naphthaldehyde,1 which can be analyzed using a fluorescence plate reader at excitation and emission wavelengths of 330 and 465 nm, respectively.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Wolf, N.M., Morisseau, C., Jones, P.D., et al. Development of a high-throughput screen for soluble epoxide hydrolase inhibition. Anal. Biochem. 355, 71-80 (2006).