For immunochemical detection of SPHK1
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Sphingosine Kinase 1 Polyclonal FITC Antibody

Item No. 10012201

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • SK1
  • SPHK1
  • SPK1
Immunogen
Synthetic peptide from an internal region of human SPHK1
Amino Acids
DLESEKYRRLG
Formulation (Request formulation change)
100 µg of peptide affinity-purified polyclonal antibody conjugated to fluorescein
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
FC and WB
Species Reactivity
(+) Human(+) Mouse(+) Pig
UniProt Accession №
Q9NYA1
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea (100 µg)

    $388

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    Product Description

    The primary use of this antibody conjugate is for the detection of SPHK1 in intact cells by direct immunolabeling methods such as flow cytometry or immunofluorescence microscopy. SPHK1 is one of the enzymes involved in sphingolipid metabolism. SPHK1 catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate. This reaction plays an important role in determining cell proliferation versus cell death.1,2 SPHK1 is found in a wide variety of tissues and cell types including kidney, liver, spleen, heart, platelets, and human tumors.3 On a cellular level, it is found in the cytosolic and membrane fractions.4 Based on the amino acid sequence, this protein has a molecular weight of approximately 43 kDa. Some reported post translational modifications may explain the shift in band migration to 50 kDa.5 NOTE: Multiple isoforms of SPHK1 are known and one is 470 amino acids. This likely explains the 50 kDa band observed.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Delon, C., Manifava, M., Wood, E., et al. Sphingosine kinase 1 is an intracellular effector of phosphatidic acid. The Journal of Biological Chemisty 279(43), 44763-44774 (2004).

    2. Merrill, A.H., Jr., Sullards, M.C., Wang, E., et al. Sphingolipid metabolism: Roles in signal transduction and disruption by fumonisins. Environ. Health Perspect. 109(suppl. 2), 283-289 (2001).

    3. Kim, J.W., Kim, Y.W., Inagaki, Y., et al. Synthesis and evaluation of sphingoid analogs as inhibitors of sphingosine kinase. Bioorg. Med. Chem. 13, 3475-3485 (2005).

    4. Maceyka, M., Sankala, H., Hait, N.C., et al. SphK1 and SphK2, sphingosine kinase isoenzymes with opposing functions in sphingolipid metabolism. The Journal of Biological Chemisty 280(44), 37118-37129 (2005).

    5. Pitson, S.M., D'Andrea, R.J., Vandeleur, L., et al. Human sphingosine kinase: Purification, molecular cloning and characterization of the native and recombinant enzymes. Biochem. J. 350(Pt 2), 429-441 (2000).