A fluorescent probe for highly reactive radicals
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APF

Item No. 10157

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]-benzoic acid
CAS Number
359010-70-1
Molecular Formula
C26H17NO5
Formula Weight
423.4
Purity
≥98%
Emission
515 nm
Excitation
490 nm
Formulation (Request formulation change)
A 5 mg/ml solution in methyl acetate
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 10 mg/mlEthanol: 10 mg/mlEthanol:PBS (pH 7.2)(1:3): .5 mg/ml
λmax
223, 278 nm
SMILES
Nc1ccc(cc1)Oc1ccc2c(c1)Oc1cc(O)ccc1C12OC(=O)c2ccccc12
InChi Code
InChI=1S/C26H17NO5/c27-15-5-8-17(9-6-15)30-18-10-12-22-24(14-18)31-23-13-16(28)7-11-21(23)26(22)20-4-2-1-3-19(20)25(29)32-26/h1-14,28H,27H2
InChi Key
SKRNNOWWEXPZBB-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    500 µg

    $171

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    1 mg

    $325

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    5 mg

    $1,023

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    Product Description

    APF is an aromatic amino-fluorescein derivative and fluorescent probe for highly reactive radicals.1 It has low intrinsic fluorescence, however, upon oxidation by hydroxyl radical, hypochlorite ion, and certain peroxidase intermediates, it is converted to the highly fluorescent molecule fluorescein, facilitating the detection of highly reactive biological radicals.1 APF is not oxidized by nitric oxide (NO), hydrogen peroxide (H2O2), or other oxidants. Fluorescein displays excitation/emission maxima of 490/515 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Setsukinai, K.i., Urano, Y., Kakinuma, K., et al. Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species. The Journal of Biological Chemisty 278(5), 3170-3175 (2003).

    Product Citations

    Pulli, B., Ali, M., Forghani, R., et al. Measuring myeloperoxidase activity in biological samples. PLoS One 8(7), e67976 (2013).