A cell permeable, fluorescent dye for detection of highly reactive oxygen species
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HPF

Item No. 10159

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(2-[6-(4'-hydroxy)phenoxy-3H-xanthene-3-on-9-yl]benzoic acid
CAS Number
359010-69-8
Molecular Formula
C26H16O6
Formula Weight
424.4
Purity
≥98%
Formulation (Request formulation change)
A 1 mg/ml solution in methyl acetate
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 20 mg/mlEthanol: 20 mg/mlEthanol:PBS (pH 7.2)(1:8): .15 mg/ml
λmax
228, 277 nm
SMILES
OC(C=C1)=CC=C1OC2=CC3=C(C=C2)C4(C(C=CC=C5)=C5C(O4)=O)C6=CC=C(O)C=C6O3
InChi Code
InChI=1S/C26H16O6/c27-15-5-8-17(9-6-15)30-18-10-12-22-24(14-18)31-23-13-16(28)7-11-21(23)26(22)20-4-2-1-3-19(20)25(29)32-26/h1-14,27-28H
InChi Key
NPOSMYDPUKBGQL-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    500 µg

    $182

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    1 mg

    $346

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    5 mg

    $1,089

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    Product Description

    HPF is a cell-permeable aromatic amino-fluorescein derivative that has little intrinsic fluorescence.1 It undergoes oxidation only by highly reactive oxygen species (hROS) such as the hydroxyl radical, peroxynitrite, and hROS generated from a peroxidase/H2O2 system. It is inert to hypochlorite ion, nitric oxide, hydrogen peroxide (H2O2), superoxide, and other oxidants. Upon oxidation, HPF is converted to the highly fluorescent molecule fluorescein, with excitation/emission maxima of 490/515 nm, respectively, allowing the simple direct detection of highly reactive biological radicals.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Setsukinai, K.i., Urano, Y., Kakinuma, K., et al. Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species. The Journal of Biological Chemisty 278(5), 3170-3175 (2003).

    Product Citations

    Pulli, B., Ali, M., Forghani, R., et al. Measuring myeloperoxidase activity in biological samples. PLoS One 8(7), e67976 (2013).