A β-oxidation enzyme
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MCAD Polyclonal Antibody

Item No. 101730

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • ACADM
  • Medium-chain Fatty Acyl-CoA Dehydrogenase
  • Medium-chain Specific Acyl-CoA Dehydrogenase mitochondrial
Immunogen
Full length human recombinant MCAD
Formulation (Request formulation change)
Protein A-purified polyclonal antibody
Storage Buffer
TBS, pH 7.4, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide
Host
Rabbit
Applications
WB; other applications not tested
Species Reactivity
(+) Human(+) Mouse(+) Ovine(+) Porcine
UniProt Accession №
P11310
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    500 µl

    $347

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    Product Description

    Medium-chain fatty acyl-CoA dehydrogenase (MCAD) is a mitochondrial enzyme that catalyzes the first step in the βoxidation of fatty acids. MCAD expression is induced during periods of fasting, when reliance on fatty acids for energy is increased.1,2 The promoter for MCAD contains a peroxisome proliferator response element (PPRE) and is regulated transcriptionally by peroxisome proliferator-activated receptor alpha (PPARα), a ligand-activated transcription factor involved in the regulation of lipid homeostasis.1,3,4 Because of this, MCAD expression can be used as a marker to evaluate the in vivo activity of PPARα.1,2 Human MCAD is approximately 87% homologous to porcine and rat MCAD, respectively.5,6 MCAD is expressed in liver, heart, kidney, and skeletal muscle.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Gulick, T., Cresci, S., Caira, T., et al. The peroxisome proliferator-activated receptor regulates mitochondrial fatty acid oxidative enzyme gene expression. Proc. Natl. Acad. Sci. USA 91(23), 11012-11016 (1994).

    2. Leone, T.C., Weinheimer, C.J., and Kelly, D.P. A critical role for the peroxisome proliferator-activated receptor α (PPARα) in the cellular fasting response: The PPARα-null mouse as a model of fatty acid oxidation disorders. Proc. Natl. Acad. Sci. USA 96(13), 7473-7478 (1999).

    3. Carter, M.E., Gulick, T., Moore, D.D., et al. A pleiotropic element in the medium-chain acyl coenzyme A dehydrogenase gene promoter mediates transcriptional regulation by multiple nuclear receptor transcription factors and defines novel receptor-DNA binding motifs. Mol. Cell. Biol. 14(7), 4360-4372 (1994).

    4. Lemberger, T., Desvergne, B., and Wahli, W. Peroxisome proliferator-activated receptors: A nuclear receptor signaling pathway in lipid physiology. Annu. Rev. Cell Dev. Biol. 12, 335-363 (1996).

    5. Kelly, D.P., Kim, J.J., Billadello, J.J., et al. Nucleotide sequence of medium-chain acyl-CoA dehydrogenase mRNA and its expression in enzyme-deficient human tissue. Proc. Natl. Acad. Sci. USA 84(12), 4068-4072 (1987).

    6. Matsubara, Y., Kraus, J.P., Ozasa, H., et al. Molecular cloning and nucleotide sequence of cDNA encoding the entire precursor of rat liver medium chain acyl coenzyme A dehydrogenase. The Journal of Biological Chemisty 262(21), 10104-10108 (1987).

    Product Citations

    Buzelle, S.L., MacPherson, R.E.K., Peppler, W.T., et al. The contribution of IL-6 to beta 3 adrenergic receptor mediated adipose tissue remodeling. Physiol. Rep. 3(2), e12312 (2015).