TP Receptor (mouse) Polyclonal Antibody

The TP receptor is a G protein-coupled receptor that mediates the action of thromboxane A₂ (TXA₂).¹ Alternative splicing of TP pre-mRNA produces two receptor variants, TP_α and TP_β, which differ in sequence and the length of their carboxy terminal tail. TP receptors are expressed primarily in platelets but are also expressed in placenta, vascular smooth muscle, brain, small intestine, thymus, kidney, lung, spleen, and monocytes.^1,2,3,4 It signals in a cell type- and expression-dependent manner and has roles in cardiovascular and hemodynamic function.^1,5 Tp^-/- mice exhibit coagulation defects, altered vascular responses to TXA₂ and arachidonic acid, and increased susceptibility to arachidonic acid-induced shock.⁶ Cayman’s TP Receptor (mouse) Polyclonal Antibody can be used for Western blot (WB). The antibody recognizes the TP_α and TP_β receptors from human, bovine, monkey, mouse, and rat samples. The apparent molecular weight for TP receptors has been reported from 37 kDa to 70 kDa, depending on different degrees of glycosylation.^7,8,9

WARNING This product is not for human or veterinary use.

1. Santilli, F., Mucci, L., and Davì, G. TP receptor activation and inhibition in atherothrombosis: the paradigm of diabetes mellitus. Intern. Emerg. Med. 6(3), 203-212 (2011).

2. Namba, T., Sugimoto, Y., Hirata, M., et al. Mouse thromboxane A₂ receptor: cDNA cloning, expression and Northern blot analysis. Biochem. Biophys. Res. Commun. 184(3), 1197-1203 (1992).

3. Båtshake, B., Nilsson, C., and Sundelin, J. Structure and expression of the murine thromboxane A₂ receptor gene. Biochem. Biophys. Res. Commun. 256(2), 391-397 (1999).

4. Mais, D.E., True, T.A., and Martinelli, M.J. Characterization by photoaffinity labelling of the human platelet thromboxane A₂/prostaglandin H₂ receptor: Evidence for N-linked glycosylation. Eur. J. Pharmacol. 227(3), 267-274 (1992).

5. Petri, M.H., Tellier, C., Michiels, C., et al. Effects of the dual TP receptor antagonist and thromboxane synthase inhibitor EV-077 on human endothelial and vascular smooth muscle cells. Biochem. Biophys. Res. Commun. 441(2), 393-398 (2013).

6. Thomas, D.W., Mannon, R.B., Mannon, P.J., et al. Coagulation defects and altered hemodynamic responses in mice lacking receptors for thromboxane A₂. J. Clin. Invest. 102(11), 1994-2001 (1998).

7. Wang, G.-R., Zhu, Y., Halushka, P.V., et al. Mechanism of platelet inhibition by nitric oxide: In vivo phosphorylation of thromboxane receptor by cyclic GMP-dependent protein kinase. Proc. Natl. Acad. Sci. USA 95(9), 4888-4893 (1998).

8. Takahashi, N., Takeuchi, K., Abe, T., et al. Immunolocalization of rat thromboxane receptor in the kidney. Endocrinology 137(11), 5170-5173 (1996).

9. Habib, A., Fitzgerald, G.A., and Maclouf, J. Phosphorylation of the thromboxane receptor α, the predominant isoform expressed in human platelets. The Journal of Biological Chemisty 274(5), 2645-2651 (1999).

McNeish, A.J., Jimenez-Altayo, F., Cottrell, G.S., et al. Statins and selective inhibition of Rho kinase protect small conductance calcium-activated potassium channel function (KCa2.3) in cerebral arteries. PLoS One 7(10), e46735 (2012).