For immunochemical detection of NAPE-PLD
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Applicable for Use with
10303NAPE-PLD Blocking Peptide (aa 159-172)
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NAPE-PLD (Internal) Polyclonal Antibody

Item No. 10305

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • N-Acyl-Phosphatidylethanolamine-Hydrolysing Phospholipase D
Immunogen
Synthetic peptide from an internal region of human protein NAPE-PLD
Formulation (Request formulation change)
500 µl of peptide affinity-purified polyclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
WB
Species Reactivity
(+) Human(+) Mouse(+) Rat
UniProt Accession №
Q6IQ20
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    1 ea (500 µl)

    $401

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    Product Description

    N-acylethanolamines (NAEs) are involved in diverse biological processes such as inflammatory regulation, apoptosis, and tissue degeneration.1 In animals, NAEs are mainly biosynthesized via a membrane phospholipid-dependent pathway, which is the enzymatic hydrolysis of N-acyl-phosphatidylethanolamine (NAPE). The enzyme catalyzing this reaction is a phospholipase D subtype selective for NAPE named N-acyl-phosphatidylethanolamine-hydrolysing phospholipase D (NAPE-PLD). It has been cloned from mouse, rat, and human and is 393-396 amino acids in length, with an estimated molecular weight of 46 kDa. Both NAPE-PLD mRNA and protein activity have been detected in a wide range of tissues with the highest levels in brain, kidney, and testis.2 In rat, NAPE-PLD activity in the brain is low in neonates and is 15-fold higher in adults, whereas the activity remains constant in the heart during development.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hansen, H.S., Moesgaard, B., Petersen, G., et al. Putative neuroprotective actions of N-acyl-ethanolamines. Pharmacol. Ther. 95(2), 119-126 (2002).

    2. Okamoto, Y., Morishita, J., Tsuboi, K., et al. Molecular characterization of a phospholipase D generating anandamide and its congeners. The Journal of Biological Chemisty 279(7), 5298-5305 (2004).

    3. Moesgaard, B., Petersen, G., Jaroszewski, J.W., et al. Age dependent accumulation of N-acyl-ethanolamine phospholipids in ischemic rat brain: A 31P NMR and enzyme activity study. J. Lipid Res. 41(6), 985-990 (2000).

    Product Citations

    Kucera, R., Bouskila, J., Elkrief, L., et al. Expression and localization of CB1R, NAPE-PLD, and FAAH in the vervet monkey nucleus accumbens. Sci. Rep. 8, 8689 (2018).

    Maccarone, R., Rapino, C., Zerti, D., et al. Modulation of type-1 and type-2 cannabinoid receptors by saffron in a rat model of retinal neurodegeneration. PLoS One 11(11), e0166827 (2016).