Purified human recombinant enzyme
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

SIRT6 (human, recombinant)

Item No. 10315

Product Insert (PDF)
Technical Information
Synonyms
  • NAD-dependent Deacetylase 6
  • Silent Information Regulator 6
  • SIR2L6
  • SIR2-like Protein 6
  • Sirtuin 6
Purity
≥80% estimated by SDS-PAGE
Source
active recombinant N-terminal His-tagged enzyme expressed in E. coli
Amino Acids
1-355
MW
43.7 kDa
Formulation (Request formulation change)
25 mM Tris-HCl, pH 8.0, with 100mM NaCl, 20% glycerol
UniProt Accession №
Q8N6T7
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    100 µg

    $489

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group.1,2,3 There are seven human sirtuins, which have been designated SIRT1-7.4 SIRT6 associates specifically with telomeres and functions at chromatin to decrease NF-κB signaling.5,6 Mammalian cells depleted of SIRT6 display abnormal telomere structures similar to defects found in Werner syndrome, a premature ageing disorder, and have a shortened life span.5,6 Since SIRT6 binds and attenuates NF-κB signaling, it is proposed that activators of SIRT6 may be effective anti-cancer and anti-inflammatory drugs and may increase longevity.5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Imai, S.I., Armstrong, C.M., Kaeberlein, M., et al. Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase. Nature 403(6771), 795-800 (2000).

    2. Tanner, K.G., Landry, J., Sternglanz, R., et al. Silent information regulator 2 family of NAD-dependent histone/protein deacetylases generates a unique product, 1-O-acetyl-ADP-ribose. Proc. Natl. Acad. Sci. USA 97(26), 14178-14182 (2000).

    3. Tanny, J.C., and Moazed, D. Coupling of histone deacetylation to NAD breakdown by the yeast silencing protein Sir2: Evidence for acetyl transfer from substrate to an NAD breakdown product. Proc. Natl. Acad. Sci. USA 98(2), 415-420 (2001).

    4. Frye, R.A. Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins. Biochem. Biophys. Res. Commun. 273(2), 793-798 (2000).

    5. Kawahara, T.L.A., Michishita, E., Adler, A.S., et al. SIRT6 links histone H3 lysine 9 deacetylation to NF- k B-dependent gene expression and organismal life span. Cell 136, 62-74 (2009).

    6. Michishita, E., McCord, R.A., Berber, E., et al. SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin. Nature 452, 492-496 (2008).