Active, pure mouse recombinant enzyme
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12-Lipoxygenase (mouse, recombinant)

Item No. 10341

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Arachidonate 12-Lipoxygenase
  • 12-LO
  • 12-LOX
Purity
≥80% estimated by SDS-PAGE
Source
Active recombinant mouse C-terminal His-tagged protein expressed in insect cells
MW
76 kDa
Formulation (Request formulation change)
50 mM sodium phosphate pH 7.2, 100 mM sodium chloride, 20 µM FeCl2, and 30% glycerol
UniProt Accession №
P39655
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    25 µg

    $402

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    Product Description

    12-Lipoxygenase (12-LO) catalyzes the formation of 12-HpETE from arachidonic acid.1 There are two main types of 12-LO, platelet-type and leukocyte-type, which share only 60% identity at the amino acid level and strongly differ in substrate specificity and enzyme kinetics.2 Leukocyte-type 12-LO has the ability to also produce 15-HpETE, however, platelet-type predominately forms 12-HpETE.2 Epidermal-type 12(S)- and 12(R)-LO enzymes have also been characterized, which catalyze the synthesis of their respective stereospecific 12-HETE products.2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Chen, X.-S., Kurre, U., Jenkins, N.A., et al. cDNA cloning, expression, mutagenesis of C-terminal isoleucine, genomic structure, and chromosomal localizations of murine 12-lipoxygenases. The Journal of Biological Chemisty 269(19), 13979-13987 (1994).

    2. McDonnell, M., Davis, W., Jr., Li, H., et al. Characterization of the murine epidermal 12/15-lipoxygenase. Prostaglandins Other Lipid Mediat. 63, 93-107 (2001).

    3. McDonnell, M., Li, H., and Funk, C.D. Characterization of epidermal 12(S) and 12(R) lipoxygenase. Eicosanoids and other bioactive lipids in cancer, inflammation and radiation injury ,5 147-153 (2002).