Active, pure human recombinant enzyme
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Sphingosine Kinase 1 (human, recombinant)

Item No. 10348

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • SK1
  • SPHK1
Purity
≥80% estimated by SDS-PAGE
Source
Active human recombinant N-terminal His-tagged protein from Sf9 cells
MW
47.5 kDa
Formulation (Request formulation change)
25 mM HEPES, pH 8.0, with 150 mM sodium chloride, 3 mM DTT, 0.05% polysorbate 20, and 25% glycerol
UniProt Accession №
Q9NYA1
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    25 µg

    $469

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    Product Description

    Sphingosine kinase (SPHK) is an important enzyme in the sphingolipid metabolic pathway. SPHKs phosphorylate D-erythro-sphingosine to yield sphingosine-1-phosphate (S1P). To date two isoforms of SPHK, SPHK1 and SPHK2, have been identified & characterized from mammalian cells.1 Among them, SPHK1 has been implicated to play a role in signaling pathways in mast cells and in TNF-α-triggered responses of fibroblasts and epithelial cells. Recent findings also indicate that SPHK1 mRNA levels are increased in a variety of human tumors. Brain tumors with high SPHK1 expression correlated with poor survival of patients and high expression of SPHK1 appears to be an oncogenic event required for erythroleukaemic progression.2,3 Thus SPHK is a potential therapeutic target for the control of cancer and inflammation.2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Pitson, S.M., D'Andrea, R.J., Vandeleur, L., et al. Human sphingosine kinase: Purification, molecular cloning and characterization of the native and recombinant enzymes. Biochem. J. 350(Pt 2), 429-441 (2000).

    2. Taha, T.A., Hannun, Y.A., and Obeid, L.M. Sphingosine kinase: Biochemical and cellular regulation and role in disease. J. Biochem. Mol. Biol. 39(2), 113-131 (2006).

    3. Leclercq, T.M., and Pitson, S.M. Cellular signalling by sphingosine kinase and sphingosine 1-phosphate. IUBMB Life 58(8), 467-472 (2006).