EP₂ Receptor Polyclonal PE Antibody

This polyclonal antibody is labeled with R-Phycoerythrin (PE) and can be used for immunofluorescent labeling of cellular EP₂ receptor following permeabilization. R-PE absorption maxima occurs at 565>540>498 nm and the emission maximum occurs at 578 nm, however standard excitation by 488 nm lasers is sufficient to generate signal detected in the PE channel of flow cytometers. The biological effects of prostaglandin E₂ (PGE₂) are mediated through interaction with four distinct membrane-bound G-protein coupled EP receptors: EP₁, EP₂, EP₃, and EP₄.^1,2 Binding of PGE₂ to the EP₂ receptor results in an increase in adenylate cyclase activity with a subsequent increase in cAMP.^3,4 Pharmacologically, EP₂ receptors mediate relaxation of smooth muscle and are distinguished from EP₄ receptors by their sensitivity to the EP₂ receptor selective agonist butaprost.^1,2,3 The human EP₂ receptor is comprised of 358 amino acids with a molecular mass of approximately 40,000.³ mRNA for the EP₂ receptor is expressed in a variety of tissues including lung, placenta, spleen, intestine, kidney, and sensory neuron.^3,4,5

WARNING This product is not for human or veterinary use.

1. Coleman, R.A., Smith, W.L., and Narumiya, S. International Union of Pharmacology classification of prostanoid receptors: Properties, distribution, and structure of the receptors and their subtypes. Pharmacol. Rev. 46(2), 205-229 (1994).

2. Coleman, R.A., Eglen, R.M., Jones, R.L., et al. Prostanoid and leukotriene receptors: A progress report from the IUPHAR working parties on classification and nomenclature. Adv. Prostaglandin Thromboxane Leukot. Res. 23, 283-285 (1995).

3. Narko, K., Saukkonen, K., Ketola, I., et al. Regulated expression of prostaglandin E₂ receptors EP₂ and EP₄ in human ovarian granulosa-luteal cells. J. Clin. Endocrinol. Metab. 86(4), 1765-1768 (2001).

4. Nemoto, K., Pilbeam, C.C., Bilak, S.R., et al. Molecular cloning and expression of a rat prostaglandin E₂ receptor of the EP₂ subtype. Prostaglandins 54(4), 713-725 (1997).

5. Southall, M.D., and Vasko, M.R. Prostaglandin receptor subtypes, EP3C and EP₄, mediate the prostaglandin E₂-induced cAMP production and sensitization of sensory neurons. The Journal of Biological Chemisty 276(19), 16083-16091 (2001).